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J Korean Med Sci 2006; 21: 1000-4 ISSN 1011-8934 Copyright The Korean Academy of Medical Sciences Cotransplanted Bone Marrow Derived Mesenchymal Stem Cells (MSC) Enhanced Engraftment of Hematopoietic
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To test this hypothesis, we treated the rat model of primary bone marrow-based (PLUS) transplantation (T1) with a single dose of 100 kg/kg (T1) versus 10 kg/kg (T2) of C57Bl/6 mice. Our data confirmed that this dose was not toxic (Fisher's exact test; p > 0.05) and the maximum survival (as determined by survival curve) was increased by 7.8% compared with T1 treatment (p > 0.05; Data not shown). The average age at which the mortality was 30% was also markedly lower during T1 versus T2 (p < 0.001). This result suggests that the T1 dose could be increased using this method to give increased survival and, in part, because of the additional use of bone marrow derived cells, an improved engraftment outcome in a rat model. To determine if the combination of bone marrow stem cells with marrow derived stem cells (MDC) in PLUS transplantation induces the same engraftment as T1/T2, we performed microchimerism assays. In the T1 model animals, 100 kg/kg, administered as a single injection, induced a substantial increase in chimer ism, with nearly 1.6 trillion, more than doubling the percentage of circulating human mesenchymal stem cells in T1 (p < 0.001). Using the same dose of marrow cells used in our earlier study, a single injection resulted in the same increase in chimeric cells which were more abundant in the circulation of T1 animals (p < 0.001) compared with T2 animals (p = 0.003). By contrast, bone marrow derived stem cells, injected as a single subcutaneous injection, failed to induce chimeric cells in circulation, although more chimeric stem cells were seen in the T2 animals. Taken together, these results suggest that marrow derived and bone marrow derived stem cells enhance engraftment of HSS, and that this enhancement is due to an enhanced engraftment of the graft.

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MSCS stands for Mesenchymal Stem Cells, which are specialized cells that can differentiate into various cell types.
There is no specific requirement to file MSCS. However, researchers and medical professionals working with MSCs may need to file certain documentation related to their research or clinical applications.
The process of filling out MSCS-related documentation may vary depending on the specific requirements set by the governing bodies or institutions. It typically involves providing information about the origin of the MSCs, their characteristics, intended use, and any relevant safety or ethical considerations.
The purpose of MSCs is to potentially serve as a regenerative therapy for various medical conditions. They have the ability to differentiate into different cell types and exhibit anti-inflammatory and immunomodulatory properties, making them promising for tissue repair and immunotherapy.
The specific information that needs to be reported on MSCS may depend on the governing regulations. Generally, it includes details about the source of the cells, their characteristics, genetic stability, quality control measures, and documentation of ethical and legal compliance.
The deadline to file MSCS-related documentation in 2023 may depend on the specific regulations or requirements in place. It is advised to consult the relevant authorities or governing bodies for the accurate deadline.
The penalties for late filing of MSCS-related documentation can vary and depend on the governing regulations and the severity of the non-compliance. It may include financial penalties, suspension of research activities, or loss of certain privileges or grants. It is important to comply with the filing deadlines to avoid any potential penalties.
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