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Sept. 18th, 2012 Title of the project: Dissecting the molecular mechanisms driving the specification and differentiation of early cell lineages of the mammalian embryo. Name of the lab: Mouse Functional
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SILENCE. The project is still in progress and the timeline is as follows. March 2016: Submitted the first project report. June 2016: Accepted the project report and started the project. August 2016: Submitted the supplementary document. October 2016: Reviewed the supplementary document and submitted the supplemental document. December 2016: Approved the revised project report. The timeline includes the following features: January 2017: First experiments for establishing the project goal. February 2017: First experimental series, to establish the hypothesis of the project. March 2017: First experimental series, to establish the cell differentiation pathway and the mechanism of genetic mutation. April 2017: First experiment to confirm the hypothesis established in the preliminary study and to establish the mechanism of genetic mutation. June 2017: First experimental series, to test the hypothesis established in the preliminary study. July 2017: Second experiment to establish the hypothesis established in the preliminary study and to establish the mechanism of genetic mutation. October 2017: Third experiment to confirm the hypothesis established in the preliminary study and to establish the mechanism of genetic mutation. December 2017: Final experiments in establishing the proposal of the project and to establish the mechanisms of genetic mutation and genetic recombination. These experiments are conducted by a dedicated group of experts comprising Cell Culture Technology experts, Biology experts, Post scientists from the French and Spanish National Institutes of Health (INSERT) and a researcher from a German research center. During these experiments, the laboratory produces the next generation of cultured embryos, that are, by definition, cell tissue of different types. The goal is to achieve these embryos with the greatest level of tissue and genetic variety, while in order to avoid the possible occurrence of any genetic defects. A technical challenge will be the generation of these tissue types in a controlled, precise and reproducible manner. During the generation of the cell cultures, the technical challenges and uncertainties are the same as for the generation of a culture without cells.

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