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Bathing. Srinivasan et al. / International Journal of Pharma Sciences and Research (IJ PSR) Spectrophotometry Determination of drugs in bulk and pharmaceutical dosage forms by using Tetracyanoethylene
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How to fill out spectrophotometric determination of drugs
How to fill out spectrophotometric determination of drugs:
01
Prepare the sample: Start by weighing or measuring the appropriate amount of the drug to be analyzed. Maintain the sample's purity and integrity throughout the process.
02
Choose a suitable solvent: Dissolve the drug in a solvent that is compatible and doesn't interfere with the analysis. Use a blank solvent as a reference to compensate for the solvent's absorbance.
03
Set up the spectrophotometer: Calibrate the spectrophotometer using appropriate standards that are similar to the drug being analyzed. Set the desired wavelength and adjust the necessary settings for accurate measurements.
04
Obtain the absorbance readings: Measure the absorbance of the drug solution using the prepared sample and the blank solvent as a reference. Record the readings accurately to provide reliable data.
05
Calculate the drug concentration: Use the Beer-Lambert law or an appropriate calibration curve to determine the drug concentration based on the absorbance readings.
06
Validate the results: Perform necessary quality control checks to ensure the accuracy and precision of the obtained results. Repeat the analysis if required.
Who needs spectrophotometric determination of drugs?
01
Pharmaceutical researchers: Spectrophotometric determination is crucial in drug development processes to analyze drug compounds, measure their concentrations, and assess their purity.
02
Quality control laboratories: Pharmaceutical companies and regulatory authorities utilize spectrophotometry to ensure the consistency, safety, and efficacy of drug formulations during manufacturing and distribution.
03
Clinical laboratories: Spectrophotometric analysis helps medical professionals monitor drug levels in patients, leading to effective dosage adjustments and improved treatment outcomes.
04
Forensic laboratories: Spectrophotometry assists in identifying and quantifying drugs in biological samples, aiding in investigations and legal proceedings.
05
Environmental laboratories: Spectrophotometric techniques are employed to detect and measure drug residuals, pollutants, and contaminants in water, soil, and other environmental samples to evaluate potential risks and human exposure.
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