Get the free Rat IL-10 ELISA KIT - Eagle Biosciences

Package Insert Rat IL10 ELISA KIT Catalog Number: IL1021K01 (1 × 96 wells) For Research Use Only. Not for use in diagnostic procedures. v. 1.0 Eagle Biosciences, Inc. 20A Northwest Blvd., Suite 112,

How to fill out rat il-10 elisa kit

How to fill out a rat IL-10 ELISA kit:

1. Carefully read and understand the instructions provided with the rat IL-10 ELISA kit. Familiarize yourself with all the components included in the kit, such as the microplate, dilution buffer, standards, and antibodies.
2. Start by preparing the samples or rat serum that you want to test for IL-10. Follow any specific instructions on how to handle or treat the samples, such as centrifuging or diluting them if necessary.
3. Prepare the microplate by adding the capture antibody to the designated wells. The specific instructions will guide you on the dilution and volume required. Incubate the plate for a recommended period to allow the antibodies to bind to the plate.
4. Assemble and label the other wells in the microplate according to the sample names or standards you will use. It is advisable to include positive and negative controls as well for accurate comparison.
5. Dilute the standards provided in the kit (usually recombinant rat IL-10 with known concentrations) using the dilution buffer in the kit. Add the appropriate volume of each standard to their respective microplate wells.
6. Take your pre-treated rat samples and properly dilute them using the dilution buffer. Add the appropriate volume of each sample to their designated wells in the microplate.
7. Incubate the microplate for a recommended period to allow IL-10 present in the samples or standards to bind with their respective antibodies.
8. After the incubation, wash the microplate thoroughly to remove any unbound substances. Follow the specified washing steps provided in the kit's instructions.
9. Add the detection antibody (usually enzyme-conjugated) to each well of the microplate, including the standard and sample wells. Incubate the plate for a recommended period to allow the detection antibody to bind to the IL-10 present in the wells.
10. Perform another washing step to remove any unbound detection antibody.
11. Add the substrate solution to the microplate wells. The substrate initiates a chemical reaction that produces a color change proportional to the amount of IL-10 present in the sample or standard.
12. Allow the reaction to occur for the specified incubation time or until the color development is sufficient. The reaction process and timing depend on the specific ELISA kit used.
13. Finally, stop the reaction by adding a stop solution or stop buffer. This step halts the enzymatic activity and stabilizes the color change.
14. Using a microplate reader, measure the absorbance or optical density of each well at the appropriate wavelength specified for the ELISA kit. Typically, the higher the absorbance, the higher the concentration of IL-10 in the sample.

Who needs a rat IL-10 ELISA kit?

1. Researchers working in the field of immunology or studying the role of IL-10 in rat models.
2. Scientists investigating inflammation, autoimmune diseases, or allergic responses in rats.
3. Pharmaceutical companies or drug developers studying the effects of potential drug candidates on IL-10 regulation in rats.
4. Biotechnology companies involved in the development of diagnostic tests or vaccines targeting IL-10 in rats.
5. Veterinarians or animal health researchers studying IL-10 levels in rats for disease diagnostics or monitoring purposes.

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