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Lab 3 Western blot Purpose The purpose of this lab is to introduce students to the utility and basic methodology of Western blotting (including fundamentals of polyacrylamide gel electrophoresis and
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01
Obtain all necessary materials and reagents for lab 3 western blot.
02
Prepare the protein samples to be used for blotting.
03
Prepare the gel for electrophoresis by mixing the appropriate components.
04
Load the protein samples onto the gel and run the gel in an electrophoresis system.
05
Transfer the proteins from the gel onto a membrane using a blotting apparatus.
06
Block the membrane to prevent non-specific binding.
07
Incubate the membrane with the primary antibody specific to the protein of interest.
08
Wash the membrane to remove any unbound primary antibody.
09
Incubate the membrane with the secondary antibody conjugated with a detection system.
10
Wash the membrane again to remove any unbound secondary antibody.
11
Visualize the protein bands using a suitable detection method.
12
Analyze the western blot results and interpret the obtained data.

Who needs lab 3 western blot?

01
Lab researchers conducting experiments to study protein expression levels.
02
Scientists investigating protein-protein interactions.
03
Diagnostic labs performing protein analysis for medical purposes.
04
Pharmaceutical companies evaluating the efficacy of drugs on protein targets.
05
Biotechnology companies developing new protein-based products.

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