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Anal Bioanal Chem (2017) 409:395410 DOI 10.1007/s0021601698806REVIEWGlycans and glycoproteins as specific biomarkers for cancer Kuchen J. Silesia 1 & Young Park 1 & Carlito B. Guerrilla 1Received:
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How to fill out glycans and glycoproteins as

01
To fill out glycans and glycoproteins, follow these steps:
02
Obtain the protein or glycoprotein of interest.
03
Identify the potential glycosylation sites on the protein or glycoprotein.
04
Determine the desired type of glycan to be attached to each glycosylation site.
05
Prepare the glycan structure by chemically synthesizing or isolating it from natural sources.
06
Activate the glycan by adding a suitable leaving group or functional group.
07
Mix the activated glycan with the protein or glycoprotein under appropriate reaction conditions.
08
Allow the glycan to attach to the glycosylation sites through enzymatic or chemical reactions.
09
Purify the resulting glycosylated protein or glycoprotein to remove any unreacted glycan or impurities.
10
Characterize the glycosylated product to confirm the successful attachment of glycans.
11
Analyze the biological activity or functionality of the glycosylated protein or glycoprotein, if necessary.

Who needs glycans and glycoproteins as?

01
Glycans and glycoproteins are needed by various individuals and industries, including:
02
- Biomedical researchers who study protein folding, structure, and function, as glycans play important roles in these processes.
03
- Pharmaceutical companies developing glycoprotein-based therapeutics, as the glycan component can affect drug efficacy, stability, and immunogenicity.
04
- Diagnostic laboratories using glycoproteins as biomarkers to detect and monitor diseases.
05
- Biotechnology companies producing glycosylated proteins for industrial applications, such as enzyme production or food processing.
06
- Glycobiologists investigating the roles of glycans in cellular processes, development, and disease.
07
- Immunologists studying the interactions between glycans and the immune system in health and disease.

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