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Bowling Green State UniversityScholarWorks BGS Biological Sciences Faculty PublicationsBiological Sciences2012Extraction of DNA from Plant and Fungus Tissues in situ Scott O. Rogers Bowling Green
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To fill out extraction of DNA from, follow these steps:
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Collect the biological sample that contains DNA, such as cells from the mouth, blood, or tissues.
03
Place the sample in a clean container, ensuring it does not come into contact with contaminants.
04
Add a cell lysis solution to break down the cell membranes and release the DNA.
05
Mix the sample and lysis solution thoroughly, ensuring proper mixing of the components.
06
Centrifuge the mixture to separate the cellular debris and other components from the DNA-containing supernatant.
07
Transfer the supernatant to a new tube, leaving behind any solid residue.
08
Add an aqueous solution, such as ethanol, to precipitate the DNA from the supernatant.
09
Mix the solution, causing the DNA to become visible as a stringy mass.
10
Use a pipette or other suitable tool to carefully collect and transfer the DNA to a clean container.
11
Wash the DNA with ethanol to remove any remaining impurities and then allow it to air dry.
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Once dry, the extracted DNA is ready to be used for various applications, such as genetic testing or research.

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Extraction of DNA is useful and necessary for various individuals or groups, including:
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- Agricultural specialists who utilize DNA extraction to develop genetically modified crops or livestock.
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- Conservationists and wildlife researchers who extract DNA to study biodiversity and population dynamics.
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- Curious individuals who want to explore their own genetic heritage through at-home DNA testing kits.

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