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SAFETY DATA SHEET Section 1 Identification Product Numeric Annexing V Apoptosis Detection Kit with Catalog No.640914Recommended Research use only. Company Street Address City, State, Zip, Country
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How to fill out cell-based annexin v fitc
How to fill out cell-based annexin v fitc
01
Thaw the annexin V binding buffer and keep it on ice.
02
Harvest the adherent cells or pellet the suspension cells and wash them with cold PBS.
03
Resuspend the cells in 1X annexin V binding buffer at a concentration of 1x10^6 cells/ml.
04
Add annexin V-FITC and PI to the cell suspension and mix gently.
05
Incubate the cells for 15 minutes at room temperature in the dark.
06
Analyze the cells using flow cytometry within 1 hour.
Who needs cell-based annexin v fitc?
01
Researchers studying apoptosis in cell culture systems.
02
Scientists investigating cell death pathways.
03
Laboratories conducting drug screening assays for apoptosis inducers.
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What is cell-based annexin v fitc?
cell-based annexin v fitc is a technique used to detect apoptotic cells by staining them with annexin V protein labeled with fluorescein isothiocyanate (FITC).
Who is required to file cell-based annexin v fitc?
Scientists and researchers conducting experiments involving cell apoptosis are required to file cell-based annexin v fitc.
How to fill out cell-based annexin v fitc?
To fill out cell-based annexin v fitc, one needs to provide information about the experiment conducted, including the cell type used, the treatment applied, and the results obtained.
What is the purpose of cell-based annexin v fitc?
The purpose of cell-based annexin v fitc is to identify and quantify the percentage of cells undergoing apoptosis in a given sample.
What information must be reported on cell-based annexin v fitc?
The information reported on cell-based annexin v fitc should include the number of apoptotic cells, the total number of cells counted, and any relevant experimental conditions.
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