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Resolution (r) = 1.22/(NA(obj) + NA(cold)) Where r is resolution (the smallest resolvable distance between two objects), NA is a general term for the microscope numerical aperture, is the imaging wavelength, NA(obj) equals the objective numerical aperture, and NA(cold) is the condenser numerical aperture.
1:44 7:52 Suggested clip Resolution of chromatography — YouTubeYouTubeStart of suggested client of suggested clip Resolution of chromatography — YouTube
Equation (1) indicates that the resolution is the difference between peak retention times divided by the average peak width. In a peak with Gaussian distribution, the peak width is W = 4 (where is the standard deviation) and the peak FHM is W0.
Resolution is calculated using the separation of two peaks in terms of their average peak width at the base (tR2 > tR1). In the case of two adjacent peaks, it may be assumed that the peak width at the base wb1 wb2, and thus, the width of the second peak may be substituted for the average value.
The resolution of an elation is a quantitative measure of how well two elation peaks can be differentiated in a chromatographic separation. It is defined as the difference in retention times between the two peaks, divided by the combined widths of the elation peaks. RS=2[(tR)B(tR)A]WB+WA.
The resolution of an optical microscope is defined as the shortest distance between two points on a specimen that can still be distinguished by the observer or camera system as separate entities.
In microscopy, the term 'resolution' is used to describe the ability of a microscope to distinguish detail. In other words, this is the minimum distance at which two distinct points of a specimen can still be seen — either by the observer or the microscope camera — as separate entities.
The resolution of a specimen viewed through a microscope can be increased by changing the objective lens. The objective lenses are the lenses that protrude downward over the specimen. Grasp the nose piece. The nose piece is the platform on the microscope to which the three or four objective lenses are attached.
Resolving power is defined as the ability of a microscope or telescope to distinguish two close together images as being separate. An example of resolving power is how well a telescope can show two stars as being separate stars.
Convert your megapixel count to a total pixel count by multiplying it by 1 million, if necessary. Multiply your pixel count by the horizontal-to-vertical ratio, then separately, by your vertical-to-horizontal ratio. Take the square root of your resulting numbers. You now have the resolution of the camera.
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