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Resolution (r) = 1.22/(NA(obj) + NA(cold)) Where r is resolution (the smallest resolvable distance between two objects), NA is a general term for the microscope numerical aperture, is the imaging wavelength, NA(obj) equals the objective numerical aperture, and NA(cold) is the condenser numerical aperture.
Equation (1) indicates that the resolution is the difference between peak retention times divided by the average peak width. In a peak with Gaussian distribution, the peak width is W = 4 (where is the standard deviation) and the peak FHM is W0. 5h = 2.354.
Resolution. The resolution of an elation is a quantitative measure of how well two elation peaks can be differentiated in a chromatographic separation. It is defined as the difference in retention times between the two peaks, divided by the combined widths of the elation peaks.
The width at half-height is determined by measuring the height of the peak crest above the baseline, dividing by two, and then measuring the span between the rising and falling sides of the peak where the signal crosses the half-height points.
In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram.
Column efficiency, indicated as the number of theoretical plates per column, is calculated as N = 5.54 (tR / w0.5)2 where tRy is the retention time of the analyte of interest and w0.5 the width of the peak at half height.
NA= n x sin Where n is the refractive index of the imaging medium and is half of the angular aperture of the objective. D= /2 NA. Where is the wavelength of light used to image a specimen. D= 2 /NA2 R= 1.22 /Naomi+Second.
Digital images consist of a small dots or pixels. The resolution refers to the number of pixels (dots) per inch (DPI). For example, if an image contains 800-by-600 pixels and has a size of 4-by-3 inches, then the resolution is 800 pixel / 4 inches = 200 DPI.
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