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I wanted to talk about the Manitou test and this is spelled ma n to X kind of interesting spelling after a French doctor who popularized it in another way you might hear this referred to as a PPD or an ST and what these things stand for is the PPD stands for purified protein derivative and actually gives us a clue as to what we're using in this test which is that we're using specifically TB protein OPA that in parentheses and the location of the test is actually also going to be kind of a clue here with ST this is a turbot keen again referring to tuberculosis tuberculous kin test this tells you where we're going to put all that protein right we're going to put it in the skin, and you may have seen this and this is kind of a picture right here someone doing the test sometimes this refers to is kind of the bubble test a lot of people say oh yeah you know my doctor you know injected some liquid in my forearm and it kind of bubbled up and this is how people usually think about this test they remember that because a very obvious visual and so what I wanted to do is kind of give you an example or a diagram of what's actually happening when you get this test done so let's imagine this is your skin layer this is also referred to as the dermis and below the dermis is some subcutaneous so below the skin subcutaneous layer and usually not later one of the most common things is fat, so I'm just going to draw that in here this is the subcutaneous fat, so the idea here is that you're basically putting a little needle in here which is what you're seeing in that picture on the right there and that needle is full of some TB protein so this purified protein derivative is in that needle is that you're going to be injected in, so you got all these little TB proteins in here and the volume you're putting in is a small volume it's about 1/10 of a milliliter so 0.1 milliliters, and you're putting it intradermally this is actually an important point it's going into that dermis layer, so its intradermal injection and what happens is that if you then let's say moments later you remove the needle you throw it away and now what you're going to notice that cause you put a little volume in there a little 0.1 MLS that is going to bubble up right because that volume is going to make the skin puff out a little but if I came back over some time this protein is kind of diffused over this liquid has been absorbed into the skin and that bubble will disappear so if you come back you might see a little of redness because of course poking the skin causes a little of redness and irritation, but the bubble will disappear over time so what are we hoping to accomplish with this test exactly let me bring up a couple of more pictures for us well this test is going to help us answer the question of has the person had prior TB exposure just remember that that's the question we're trying to answer have they had prior TB exposure yes or no and let's think about what would happen in either...
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