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5 l of 1 10 dilution Primer 5 OR 3 3pmol/ l starting conc n 11. 5 l ddH2O 24 l Total in a 1. 5mL tube Go To http //fgc.urmc.rochester. Sequencing Protocol Reaction mix should contain 600-1000ng plasmid DNA. Miniprep DNA is 80ng/ l* 8pmol primer ddH20 to bring total up to 24 l Reaction mix to be submitted if using already reconsituted primers pGEX 5p sequencing or pGEX 3p sequencing which are at a 30pmoles/ l Miniprep DNA 10 l 2. edu/ Click on Connect to dnaLIMS Click on Login to LIMS Login...
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