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(19) & (11) EP 2 360 167 A1 EUROPEAN PATENT APPLICATION (12) (43) Date of publication: (51) Int Cl.: C07H 21/00 (2006.01) 24.08.2011 Bulletin 2011/34 (21) Application number: 10001122.0 (22) Date

How to fill out post-synformtic modification of nucleic

How to fill out post-synformtic modification of nucleic?

1. Start by gathering all the necessary materials and reagents needed for the modification process. This may include the nucleic acid sample, the modification enzyme or chemical, buffer solutions, and other required components.
2. Prepare the nucleic acid sample by isolating it from the source material, such as DNA or RNA extraction. Ensure the sample is pure and free from contaminants that may interfere with the modification process.
3. Design the specific modification to be introduced into the nucleic acid. This could involve selecting the desired modification type, such as methylation or phosphorylation, and determining the modification site(s) on the nucleic acid sequence.
4. Determine the appropriate reaction conditions for the modification. This includes optimizing factors such as temperature, pH, and reaction time to ensure efficient and specific modification of the nucleic acid.
5. Set up the modification reaction by mixing the nucleic acid sample with the modification enzyme or chemical, along with any required co-factors or activators. Follow the recommended protocols and guidelines provided by the manufacturer or established research methods.
6. Incubate the reaction mixture under the specified conditions. This allows the modification enzyme or chemical to catalyze the desired modification at the targeted sites on the nucleic acid sequence. The reaction time may vary depending on the specific modification and experimental requirements.
7. After the modification reaction is complete, the modified nucleic acid can be analyzed and characterized. This may involve techniques such as gel electrophoresis, chromatography, or mass spectrometry, to verify the presence and extent of the desired modification.

Who needs post-synformtic modification of nucleic?

1. Researchers studying epigenetics: Post-synthetic modification of nucleic acids plays a crucial role in the field of epigenetics, which investigates heritable changes in gene expression that do not involve alterations to the underlying DNA sequence. Researchers studying DNA methylation, histone modifications, or other epigenetic modifications often require post-synthetic modification of nucleic acids for their studies.
2. Biotechnologists and genetic engineers: Post-synthetic modification of nucleic acids is essential in various biotechnological and genetic engineering applications. By modifying nucleic acids, scientists can introduce specific traits or functions into organisms, develop novel gene therapy techniques, or optimize gene expression for bioproduction purposes.
3. Drug discovery and development: Post-synthetic modification of nucleic acids can be used in drug discovery and development processes. By modifying the structure or properties of nucleic acids, researchers can investigate the effects of specific modifications on drug efficacy, delivery, or stability. This information is valuable for developing nucleic acid-based therapies or designing drugs that target specific nucleic acid sequences.

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What is post-synformtic modification of nucleic?

Post-synthetic modification of nucleic acids involves adding new chemical groups or functionalizing existing groups on the nucleic acid molecule after its synthesis.

Who is required to file post-synformtic modification of nucleic?

Researchers, scientists, or individuals conducting experiments or studies involving nucleic acids may be required to file post-synthetic modifications.

How to fill out post-synformtic modification of nucleic?

To fill out post-synthetic modification of nucleic acids, one must document the specific modifications made to the nucleic acid sequence or structure, along with any relevant experimental protocols.

What is the purpose of post-synformtic modification of nucleic?

The purpose of post-synthetic modification of nucleic acids is to alter or enhance the properties or functions of the nucleic acid molecule for various research or practical applications.

What information must be reported on post-synformtic modification of nucleic?

The information reported on post-synthetic modification of nucleic acids may include details on the type of modification, the location within the nucleic acid sequence, the experimental methods used, and any observed effects.

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