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Get the free Human Liver Microsomes (HLMs) 50-Donor Pool

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Approved and current. Effective starting 9/25/2023. COA452117/452118 (version 6.0) UltraPool HLM 150 Discovery Life Sciences 6 Henshaw Street Woburn, MA 01801 Tel: (866) 8382798 info@dls.com https://www.dls.com/UltraPool HLM 150Catalog No. Lot No. Qty./PackageAssay ResultsProtein Content Storage Conditions Release Date Expiration Date452117 38300 0.5 mL 20 mg/mL in 250 mM sucrose Store at 80C 2024 April 2033 NovemberEnzyme Measured Total P450 OR Cytochrome b5 CYP1A2 CYP2A6 CYP2B6 CYP2C8...
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How to fill out human liver microsomes hlms

01
Gather necessary materials: You will need human liver microsomes, a microcentrifuge, pipettes, and any required reagents.
02
Prepare your samples by thawing and gently mixing the human liver microsomes if they are stored frozen.
03
Determine the desired concentration of microsomes for your experiment.
04
Use a pipette to transfer the appropriate volume of human liver microsomes into a clean microcentrifuge tube.
05
Add the necessary buffer solution or reagents as specified in your experimental protocol.
06
Mix the contents gently to ensure a homogenous solution.
07
If needed, incubate the mixture at the required temperature for the specified duration.
08
Proceed with downstream assays or experiments as outlined in your protocol.

Who needs human liver microsomes hlms?

01
Researchers in pharmacology and toxicology who conduct drug metabolism studies.
02
Pharmaceutical companies developing new medications that require understanding of liver metabolism.
03
Academic institutions focused on liver biology or drug interactions.
04
Regulatory agencies evaluating the safety and efficacy of new compounds.
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Human liver microsomes (HLMs) are subcellular fractions obtained from human liver tissue, containing enzymes involved in drug metabolism, particularly cytochrome P450 enzymes.
Researchers and pharmaceutical companies conducting drug metabolism studies are required to file information on human liver microsomes (HLMs) for studies involving drug development or safety assessments.
To fill out HLMs documentation, gather data on the source of liver microsomes, experimental conditions, and analytical methods used in the metabolic studies, following the specific guidelines provided by regulatory agencies.
The purpose of human liver microsomes (HLMs) is to study the metabolism of drugs in the human body, enabling researchers to understand how drugs are processed and to assess their potential effects and interactions.
Information such as the donor's age, sex, medical history, and the specific metabolic parameters observed during the study (such as enzyme activity and substrate specificity) must be reported on HLMs documentation.
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