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Nucleic Acids Research, 2024, 119 https://doi.org/10.1093/nar/gkae142 Synthetic Biology and BioengineeringJulien Capin1 , , Alexandra Harrison1 , , Renata A. Raele1 , Sathish K.N. Yadav1 , Dominique Baiwir 2 , Gabriel Mazzucchelli3 , Loic Quinton3 , Timothy J. Satchwell1 , Ashley M. Toye1 , Christiane Schaffitzel 1 , Imre Berger 1 ,4 ,5 ,*, and Francesco Aulicino1 , ,*1School of Biochemistry, University of Bristol, 1 Tankards Close, Bristol BS8 1TD, UK GIGA Proteomics
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Template-independent synformsis is a molecular process that allows the synthesis of nucleic acid sequences without a specific template, particularly at the 3-end of the molecule.
Researchers and professionals in the fields of molecular biology, genetics, and biotechnology are typically required to file data related to template-independent synformsis and 3-end in their studies and experiments.
To fill out the template-independent synformsis and 3-end documentation, one should accurately record the experimental conditions, reagents used, reaction times, and any specific observations related to the synthesis process.
The purpose of template-independent synformsis and 3-end is to facilitate the understanding of nucleic acid synthesis mechanisms and to contribute to advances in molecular biology applications such as gene expression analysis and synthetic biology.
The information that must be reported includes the specific sequence synthesized, the methods used for synthesis, any deviations from standard protocols, experimental conditions, and results obtained from the experiment.
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