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ARTICLE https://doi.org/10.1038/s41467019108461OPENA genetically encoded probe for imaging nascent and mature HAtagged proteins in vivo1234567890():,;Ning Zhao1, Kouta Kamijo2, Philip D. Fox 1, Haruka Oda3, Tatsuya Morisaki Hiroshi Kimura 2,3,4 & Timothy J. Stasevich1,41,Yuko Sato2,3,To expand the toolbox of imaging in living cells, we have engineered a singlechain variable fragment binding the linear HA epitope with high afnity and specicity in vivo. The resulting probe,

How to fill out a genetically encoded probe

How to fill out a genetically encoded probe

1. Begin by selecting the specific gene or genetic sequence you want to encode.
2. Design a DNA construct that includes the genetic sequence of interest along with a promoter region.
3. Incorporate a fluorescent protein or another reporter gene into the construct to visualize the probe's expression.
4. Ensure appropriate regulatory elements are included for expression in the target organism or cell type.
5. Clone the construct into a suitable vector using standard molecular biology techniques.
6. Transform the vector into host cells, such as bacteria or yeast, to amplify the DNA.
7. Purify the DNA from the host cells using a plasmid extraction method.
8. Assess the quality and concentration of the purified probe using techniques like gel electrophoresis and spectrophotometry.
9. Introduce the probe into the target cells through methods such as transfection, electroporation, or viral delivery.
10. Confirm successful expression of the probe in the target cells through imaging or other functional assays.

Who needs a genetically encoded probe?

1. Researchers working in molecular biology and genetics.
2. Scientists studying cellular processes and protein interactions.
3. Laboratories involved in drug discovery and development.
4. Educational institutions for teaching purposes in genetics and molecular biology courses.
5. Biotechnological companies focused on developing genetic therapies or diagnostics.

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What is a genetically encoded probe?

A genetically encoded probe is a molecular tool that is designed to specifically bind to or interact with a target molecule, such as a protein, messenger RNA, or other biomolecule, often used in biological research to visualize or measure cellular processes.

Who is required to file a genetically encoded probe?

Researchers, institutions, or companies that develop or utilize genetically encoded probes, particularly in the context of regulatory compliance or patent filings, may be required to file documentation regarding their use.

How to fill out a genetically encoded probe?

To fill out a genetically encoded probe form, one must provide specific information including the probe's identification, target interaction, experimental conditions, and any associated genetic sequences or constructs.

What is the purpose of a genetically encoded probe?

The purpose of a genetically encoded probe is to enable researchers to study, visualize, or quantify specific biological interactions, cellular processes, or molecular dynamics within living organisms.

What information must be reported on a genetically encoded probe?

Information that must be reported includes the probe's name, sequence, target specificity, experimental setup, intended use, and any relevant safety or regulatory information.