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The limit of resolution (or resolving power) is a measure of the ability of the objective lens to separate in the image adjacent details that are present in the object. It is the distance between two points in the object that are just resolved in the image.
The primary factor in determining resolution is the objective numerical aperture, but resolution is also dependent upon the type of specimen, coherence of illumination, degree of aberration correction, and other factors such as contrast-enhancing methodology either in the optical system of the microscope or in the
The resolution of an optical microscope is defined as the shortest distance between two points on a specimen that can still be distinguished by the observer or camera system as separate entities.
The resolution of a specimen viewed through a microscope can be increased by changing the objective lens. The objective lenses are the lenses that protrude downward over the specimen. Grasp the nose piece. The nose piece is the platform on the microscope to which the three or four objective lenses are attached.
Light Wavelength and Refractive Index Microscope resolution is also impacted by the wavelength of light being used to illuminate the specimen. Longer wavelengths of light offer less resolution than short wavelength illumination. As light slows down the wavelength gets shorter and yields better resolution.
The resolution of the light microscope cannot be small than the half of the wavelength of the visible light, which is 0.4-0.7 µm. When we can see green light (0.5 Am), the objects which are, at most, about 0.2 Am. Below this point, light microscope is not useful, as wavelength smaller than 400 nm is needed.
The resolution of the light microscope cannot be small than the half of the wavelength of the visible light, which is 0.4-0.7 µm. When we can see green light (0.5 Am), the objects which are, at most, about 0.2 Am. Below this point, light microscope is not useful, as wavelength smaller than 400 nm is needed.
The limit of resolution (or resolving power) is a measure of the ability of the objective lens to separate in the image adjacent details that are present in the object. It is the distance between two points in the object that are just resolved in the image. Thus, an optical system cannot form a perfect image of a point.
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