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John L
2014-05-11
It has allowed me to "type" forms that I would have otherwise needed to hand write or type on an old fashioned typewriter (which I still have). I didn't like the way the numeric columns lined up. I didn't see a way to insure that they were all right aligned.
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2016-07-01
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What is the packing material used in most packed GC columns? The packing material most often used is diatomaceous earth particles having diameters ranging from 250 to 170 um or 170 to 149 um.
There are two general types of column, packed and capillary (also known as open tubular). Packed columns contain a finely divided, inert, solid support material (commonly based on diatomaceous earth) coated with liquid stationary phase.
There are two general types of column, packed and capillary (also known as open tubular). Packed columns contain a finely divided, inert, solid support material (commonly based on diatomaceous earth) coated with liquid stationary phase.
Materials. Liquid chromatography: Traditional chromatography columns were made of glass. Modern columns are mostly made of borosilicate glass, acrylic glass or stainless steel. To prevent the stationary phase from leaking out of the column interior a polymer, stainless steel or ceramic net is usually applied.
Capillary columns are gas chromatography (GC) columns that have the stationary phase coating their inner surfaces rather than being packed into the cavity. Capillary GC columns are used to analyze samples for the individual chemical compounds that they contain.
A chromatography detector is a device used in gas chromatography (GC) or liquid chromatography (LC) to detect components of the mixture being eluted off the chromatography column. There are two general types of detectors: destructive and non-destructive.
Materials. Liquid chromatography: Traditional chromatography columns were made of glass. Modern columns are mostly made of borosilicate glass, acrylic glass or stainless steel. To prevent the stationary phase from leaking out of the column interior a polymer, stainless steel or ceramic net is usually applied.
A gas encounters less resistance in the GC column which permits use of longer column lengths. Sample stability samples analyzed by HPLC are generally thermally labile, so they are in liquid phase in the HPLC column at room temperatures.
When choosing a column, consider the polarity of both the stationary phase and your target analytes. If the stationary phase and analyte polarities are similar, then the attractive forces are strong and more retention will result.
The main difference between packed column and capillary column is that, in a packed column, the stationary phase is packed into the cavity of the column whereas, in a capillary column, the stationary phase coats the inner surface of the cavity of the column.
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