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Resolution is measured by calculating the number of dots on a line and then multiplying by the number of lines on the screen. Measure the horizontal and vertical pixels on a screen to determine resolution with tips from a tutor in this free video on resolution.
1:44 7:52 Suggested clip Resolution of chromatography — YouTubeYouTubeStart of suggested client of suggested clip Resolution of chromatography — YouTube
1:19 7:52 Suggested clip Resolution of chromatography — YouTubeYouTubeStart of suggested client of suggested clip Resolution of chromatography — YouTube
Equation (1) indicates that the resolution is the difference between peak retention times divided by the average peak width. In a peak with Gaussian distribution, the peak width is W = 4 (where is the standard deviation) and the peak FHM is W0.5h = 2.354.
The resolution of an elation is a quantitative measure of how well two elation peaks can be differentiated in a chromatographic separation. It is defined as the difference in retention times between the two peaks, divided by the combined widths of the elation peaks.
Column efficiency calculation Column efficiency, indicated as the number of theoretical plates per column, is calculated as N = 5.54 (tR / w0.5)2 where tRy is the retention time of the analyte of interest and w0.5 the width of the peak at half height.
The capacity factor (also called “capacity ratio”) is symbolized by k' (USP terminology) or k (IUPAC/ASTM terminology). It is a measure of the retention of a peak that is independent of column geometry or mobile phase flow rate. The capacity factor is calculated as: k' = (tR — t0)/t0.
Take the value in the “Width (mm)” column and divide it by the width in pixels. This is called “pixel size x”. 2. Take the value in the “Height (mm)” column and divide it by the height in pixels.
480p = 338,000 pixels / frame. 720p = 922,000 pixels / frame. 1080i = 1,037,000 pixels / frame. 1080p = 2,074,000 pixels / frame.
Look at the first number. If it's 1920 then you have a 1080p movie. If it's 1280 then you have a 720p movie.
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