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In a compound microscope, the wavelength of the light waves that illuminate the specimen limits the resolution. The wavelength of visible light ranges from about 400 to 700 nanometers. The best compound microscopes cannot resolve parts of a specimen that are closer together than about 200 nanometers.
The resolution of a specimen viewed through a microscope can be increased by changing the objective lens. The objective lenses are the lenses that protrude downward over the specimen. Grasp the nose piece. The nose piece is the platform on the microscope to which the three or four objective lenses are attached.
To achieve the maximum (theoretical) resolution in a microscope system, each of the optical components should be of the highest NA available (taking into consideration the angular aperture). In addition, using a shorter wavelength of light to view the specimen will increase the resolution.
A simple answer is that resolution of an optical system is its ability to separate two closely spaced objects. Resolution In general as the magnification increases the resolution increases, however, this much more complicated than it seems.
The maximum magnification of light microscopes is usually ×1500, and their maximum resolution is 200 nm, due to the wavelength of light. An advantage of the light microscope is that it can be used to view a variety of samples, including whole living organisms or sections of larger plants and animals.
The resolution of an optical microscope is defined as the shortest distance between two points on a specimen that can still be distinguished by the observer or camera system as separate entities.
The primary factor in determining resolution is the objective numerical aperture, but resolution is also dependent upon the type of specimen, coherence of illumination, degree of aberration correction, and other factors such as contrast-enhancing methodology either in the optical system of the microscope or in the
The limit of resolution (or resolving power) is a measure of the ability of the objective lens to separate in the image adjacent details that are present in the object. It is the distance between two points in the object that are just resolved in the image.
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