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Agent J&W DB-5 is non-polar and low bleed, available in a range of column dimensions. DB-5 is also bonded, cross-linked and solvent-rinsable and has a high temperature limit. It is equivalent to USP phase G27.
When choosing a column, consider the polarity of both the stationary phase and your target analytes. If the stationary phase and analyte polarities are similar, then the attractive forces are strong and more retention will result.
Basically, there are two main types of columns for the gas chromatography. These are so-called packed columns, which are characterized by their robustness and higher capacity. Capillary columns are used in most applications nowadays because of their high separation efficiency.
There are two general types of column, packed and capillary (also known as open tubular). Packed columns contain a finely divided, inert, solid support material (commonly based on diatomaceous earth) coated with liquid stationary phase.
Capillary columns are gas chromatography (GC) columns that have the stationary phase coating their inner surfaces rather than being packed into the cavity. Capillary GC columns are used to analyze samples for the individual chemical compounds that they contain.
The FID is the most common type of GC detector (universal detector).
Capillary columns are gas chromatography (GC) columns that have the stationary phase coating their inner surfaces rather than being packed into the cavity. Capillary GC columns are used to analyze samples for the individual chemical compounds that they contain.
The sample being measured is injected into the carrier gas using a syringe and instantly vaporizes (turns into gas form). The gases that make up the sample separate out as they move along the column (orange), which contains the stationary phase (typically, it's a thin coating on the inside wall of the column).
Materials. Liquid chromatography: Traditional chromatography columns were made of glass. Modern columns are mostly made of borosilicate glass, acrylic glass or stainless steel. To prevent the stationary phase from leaking out of the column interior a polymer, stainless steel or ceramic net is usually applied.
The main difference between packed column and capillary column is that, in a packed column, the stationary phase is packed into the cavity of the column whereas, in a capillary column, the stationary phase coats the inner surface of the cavity of the column.
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