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Transformation is the process by which an organism acquires exogenous DNA. Artificial transformation encompasses a wide array of methods for inducing uptake of exogenous DNA. In cloning protocols, artificial transformation is used to introduce recombinant DNA into host bacteria (E. Coli).
Bacteria can take up foreign DNA in a process called transformation. Transformation is a key step in DNA cloning. It occurs after restriction digest and ligation and transfers newly made plasmids to bacteria. Bacteria with a plasmid are antibiotic-resistant, and each one will form a colony.
In molecular biology and genetics, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through the cell membrane(s).
Transformation is a key step in DNA cloning. It occurs after restriction digest and ligation and transfers newly made plasmids to bacteria. Colonies with the right plasmid can be grown to make large cultures of identical bacteria, which are used to produce plasmid or make protein.
By exposing cells to a sudden increase in temperature, or heat shock, a pressure difference between the outside and the inside of the cell is created, that induces the formation of pores, through which super coiled plasmid DNA can enter.
Transformation of cells is a widely used and versatile tool in genetic engineering and is of critical importance in the development of molecular biology. The purpose of this technique is to introduce a foreign plasmid into bacteria, the bacteria then amplifies the plasmid, making large quantities of it.
Key steps in the process of bacterial transformation: (1) competent cell preparation, (2) transformation of cells, (3) cell recovery, and (4) cell plating.
Key points: Bacteria can take up foreign DNA in a process called transformation. Bacteria with a plasmid are antibiotic-resistant, and each one will form a colony. Colonies with the right plasmid can be grown to make large cultures of identical bacteria, which are used to produce plasmid or make protein.

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