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Since the limit of resolving power of a microscope is fixed by the structure of light, the shortest wavelength of visible light will give the maximum resolution. Among yellow, green, red and blue light color. Blue (500 nm) have the shortest wavelength so, it will give the best resolution.
The greatest resolving power in optical microscopy is realized with near-ultraviolet light, the shortest effective imaging wavelength. Near-ultraviolet light is followed by blue, then green, and finally red light in the ability to resolve specimen detail.
Near-ultraviolet light has the shortest usable wavelength and offers the greatest resolution. Following near-ultraviolet in descending order of wavelength are red, orange, yellow, green, blue and violet. The range in nanometers of the wavelength of the visible light is from 380 nm to 750 nm.
The maximum magnification of light microscopes is usually ×1500, and their maximum resolution is 200 nm, due to the wavelength of light. An advantage of the light microscope is that it can be used to view a variety of samples, including whole living organisms or sections of larger plants and animals.
The resolution of a specimen viewed through a microscope can be increased by changing the objective lens. The objective lenses are the lenses that protrude downward over the specimen. Grasp the nose piece. The nose piece is the platform on the microscope to which the three or four objective lenses are attached.
The resolution of a specimen viewed through a microscope can be increased by changing the objective lens. The objective lenses are the lenses that protrude downward over the specimen. Grasp the nose piece. The nose piece is the platform on the microscope to which the three or four objective lenses are attached.
The resolution of the light microscope cannot be smaller than the half of the wavelength of the visible light, which is 0.4-0.7 µm. When we can see green light (0.5 Am), the objects which are, at most, about 0.2 Am. Below this point, light microscope is not useful, as wavelength smaller than 400 nm is needed.
In order to increase the resolution (d=/2 NA), the specimen must be viewed using either shorter wavelength () light or through an imaging medium with a relatively high refractive index or with optical components which have a high NA (or, indeed, a combination of all of these factors).
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