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Pan Cytokeratin AE1/AE3 Concentrated and Predicted Antibody Cocktail Control Number: 901-011-071814 Catalog Number: CM 011 A, B, C PM 011 AA, H IPI 011 G10 Description: 0.1, 0.5, 1.0 ml, concentrated
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How to fill out pan cytokeratin ae1ae3
How to fill out pan cytokeratin ae1ae3?
01
Begin by carefully reading the instructions provided with the pan cytokeratin ae1ae3 kit. Familiarize yourself with the materials required and any specific steps or techniques mentioned.
02
Prepare the sample for testing. This may involve obtaining a tissue or cell specimen and ensuring it is properly fixed, embedded, or prepared according to the recommended protocols. Follow the appropriate guidelines for your specific sample type.
03
Retrieve the pan cytokeratin ae1ae3 kit and ensure it is within its expiration date. Open the kit and gather all necessary components, including the primary and secondary antibodies, buffers, and detection systems.
04
Apply the primary antibody to the specimen. Use the recommended dilution and incubation time provided by the manufacturer. Ensure proper coverage of the sample surface and incubate as instructed.
05
After the primary antibody incubation, thoroughly wash the sample with the provided buffer solution or any other suitable wash solution. This step is crucial to remove any unbound primary antibody and reduce background noise.
06
Proceed with the secondary antibody application. Again, follow the manufacturer's instructions for the appropriate dilution and incubation time. The secondary antibody should be specific to the primary antibody used and allow for detection of the target protein.
07
Following the secondary antibody incubation, wash the sample again to remove any unbound secondary antibody.
08
If necessary, proceed with additional detection steps, such as using an enzyme-based substrate or fluorescence-based system. Follow the kit instructions to ensure proper visualization and detection of the pan cytokeratin ae1ae3 protein.
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Finally, assess the results of the pan cytokeratin ae1ae3 staining using an appropriate detection method, such as microscopy or imaging systems. Evaluate the presence and localization of the protein in the sample.
Who needs pan cytokeratin ae1ae3?
01
Pan cytokeratin ae1ae3 is commonly used in pathology and histology laboratories for immunohistochemistry (IHC) staining procedures.
02
Pathologists and researchers may need pan cytokeratin ae1ae3 to identify and characterize various types of epithelial cells in tissue samples. It can facilitate the differentiation and classification of different types of carcinomas, including lung, breast, ovarian, and gastrointestinal cancers.
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Translational research and clinical studies often utilize pan cytokeratin ae1ae3 to evaluate the epithelial component of tumors and assess their differentiation status. This information can aid in determining prognosis, treatment options, and therapeutic response.
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Moreover, pan cytokeratin ae1ae3 staining can be helpful in distinguishing epithelial cells from other cell types or in identifying metastatic carcinomas in lymph nodes or distant organs.
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Overall, anyone involved in the field of pathology, histology, or cancer research may require pan cytokeratin ae1ae3 for immunohistochemical staining to accurately characterize and analyze epithelial cells and tumors.
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