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Quick Wizard Genomic DNA Purification Kit PROTOCOL INSTRUCTIONS FOR USE OF PRODUCTS A1120 A1123 A1125 AND A1620. Isolation of Genomic DNA from Whole Blood Cell 3ml 9ml As little as 20 l can be processed with this system* Please see Technical Manual TM050 Section 3. C. Red Blood Cell Lysis 1. Using volumes from the table above combine the appropriate volumes of Cell Lysis Solution and blood. Mix by inversion* 2. Incubate for 10 minutes at room temperature. 3. Centrifuge 300 l sample 13 000 16...
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How to fill out promega dna extraction kit
How to fill out promega dna extraction kit:
01
Prepare the sample: Collect the biological sample containing DNA and ensure it is properly labeled and stored.
02
Extraction reagent preparation: Prepare the extraction reagents following the instructions provided with the promega dna extraction kit.
03
Sample lysis: Add the biological sample to the lysis buffer and mix gently. Incubate the mixture at a specific temperature for a designated time to lyse the cells and release the DNA.
04
Protein precipitation: Add the protein precipitation solution to the lysed sample and mix thoroughly. This step helps to remove proteins and other cellular debris from the DNA solution.
05
DNA precipitation: Add the appropriate volume of isopropanol to the lysed sample and mix gently. The DNA will precipitate out of solution as a visible white pellet.
06
DNA purification: Carefully aspirate the supernatant, being cautious not to disturb the DNA pellet. Wash the DNA pellet with a designated wash solution to remove impurities.
07
DNA resuspension: Add a suitable volume of DNA resuspension solution or water to dissolve the DNA pellet, ensuring the DNA is fully rehydrated.
08
Storage: Store the extracted DNA in appropriate storage conditions, such as a freezer, to maintain its stability until further use.
Who needs promega dna extraction kit:
01
Researchers: Scientists and researchers working in various fields, such as genetics, genomics, forensics, and molecular biology, may need the promega dna extraction kit to isolate DNA from various samples for further analysis and experimentation.
02
Clinical laboratories: Clinical laboratories and hospitals may require the promega dna extraction kit for diagnostic purposes, such as identifying genetic mutations, performing genetic testing, or studying genetic diseases.
03
Educational institutions: DNA extraction experiments are often performed in educational settings, such as high schools and universities, to teach students about DNA structure and extraction techniques. The promega dna extraction kit can be useful in these educational settings.
04
Forensic laboratories: Law enforcement agencies and forensic laboratories may utilize the promega dna extraction kit to extract and analyze DNA from crime scene samples, such as blood, saliva, or hair, to aid in criminal investigations and identifications.
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What is promega dna extraction kit?
The Promega DNA extraction kit is a laboratory product designed to isolate and purify DNA from various biological samples, including tissues, blood, and cells, for use in molecular biology applications.
Who is required to file promega dna extraction kit?
Individuals or laboratories conducting DNA extraction and analysis, such as researchers, forensic scientists, and clinical laboratories, are typically the users of the Promega DNA extraction kit.
How to fill out promega dna extraction kit?
To fill out the Promega DNA extraction kit, users should follow the product's protocol, which includes inputting sample information, processing conditions, and any relevant notes about the samples being processed.
What is the purpose of promega dna extraction kit?
The purpose of the Promega DNA extraction kit is to efficiently and effectively extract high-quality DNA from samples for subsequent applications such as PCR, sequencing, and genotyping.
What information must be reported on promega dna extraction kit?
Information that must be reported when using the Promega DNA extraction kit includes sample identification, date of extraction, batch number, operator's name, and any deviations from the protocol used.
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