Get the free APHIS-Approved Laboratories Johne's Disease Serologic Tests - aphis usda

This document lists laboratories approved by APHIS for conducting serologic tests related to Johne's Disease, including contact information and laboratory capabilities.

How to fill out aphis-approved laboratories johnes disease

How to fill out APHIS-Approved Laboratories Johne's Disease Serologic Tests

1. Obtain the APHIS-Approved Laboratories Johne's Disease Serologic Tests form.
2. Fill in the laboratory name and contact information at the top of the form.
3. Provide the date of sample collection.
4. Enter the identification details for each animal being tested, including species, breed, age, and ID number.
5. Indicate the type of test being performed (i.e., ELISA, AGID).
6. Collect and package samples according to the laboratory's instructions to prevent contamination.
7. Include any required fees with the submission, if applicable.
8. Review the completed form for any missing information and ensure all details are accurate.
9. Send the completed form along with the samples to the designated APHIS-approved laboratory.
10. Retain a copy of the completed form for your records.

Who needs APHIS-Approved Laboratories Johne's Disease Serologic Tests?

1. Veterinarians who are diagnosing Johne's Disease in livestock.
2. Livestock producers seeking to test their herds for Johne's Disease.
3. State and federal animal health officials monitoring Johne's Disease in cattle populations.
4. Research institutions studying Johne's Disease and its impact on animal health.

People Also Ask about

What is the ELISA test for Johne's disease?

TEST: Johnes Antibody ELISA This is a serology test to determine if the animal has an antibody level consistent with infection by M. paratuberculosis. This test will not determine if the animal is shedding the bacteria. Faecal culture is currently the most sensitive test to determine whether bacteria are being shed.

What is the test for diagnosis of paratuberculosis?

Diagnosis of paratuberculosis is made on clinical grounds confirmed by the demonstration of MAP in the faeces by microscopy, culture, or by the use of DNA probes and the polymerase chain reaction (PCR).

What is the Johne's test for goats?

Our recommended test for screening for MAP infection in clinical or non-clinical goats is the Johne's fecal PCR test. It is highly sensitive and specific in shedding animals, but may result in false negatives during early infection prior to the onset of shedding or during intermittent shedding periods.

How much does it cost to test for Johne's disease?

Ruminant Tests and Fees Abortion PanelAnatomic Pathology$200.00 (10+) $8.25 each Iron Toxicology $30.00 Johne's Disease ELISA (10+) $8.50 each / (50+) $6.00 each Serology $10.00 Johne's Disease PCR Molecular Diagnostics $33.00 Johne's Pooled PCR Molecular Diagnostics $46.00151 more rows

What are the diagnostic tests for Johne's disease?

Available Johne's disease tests: Johne's Antibody ELISA (SES-1059) Sample: 0.5 ml serum (red top) or 0.5 ml whole blood (purple top) Cost: $5.00. Johne's Fecal PCR (BMB-1200) Sample: Fresh feces (5 gms) Cost: $32.00. Laboratory can pool up to 5 samples for $32.00.

What is the fecal test for Johne's?

The Johne's Direct Fecal PCR test is a real-time polymerase chain reaction assay with an internal amplification standard control for every sample. It is validated for high throughput in a 96-well plate format. It detects amplified DNA of Mycobacterium avium subsp.

What is the difference between PCR and ELISA in Johne's?

ELISA is inexpensive and can detect animals at a higher risk of advanced infection. Fecal PCR is used to further assess shedding of M. ptb in those individuals.

What is a differential diagnosis for Johne's disease?

Differential diagnoses include internal parasites, undernutrition, poor dentition, and internal abscesses from cheesy gland or following rumenitis. Caprine arthritis and encephalitis (CAE) is an additional differential diagnosis in goats. With many of these differentials there is often some level of inappetence.

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What is APHIS-Approved Laboratories Johne's Disease Serologic Tests?

APHIS-Approved Laboratories Johne's Disease Serologic Tests are diagnostic tests approved by the Animal and Plant Health Inspection Service (APHIS) for detecting antibodies related to Johne's disease in animals, particularly in cattle. These tests help identify animals infected with Mycobacterium avium paratuberculosis, the causative agent of Johne's disease.

Who is required to file APHIS-Approved Laboratories Johne's Disease Serologic Tests?

Individuals or entities involved in the breeding, export, or sale of livestock, especially cattle, are required to file APHIS-Approved Laboratories Johne's Disease Serologic Tests as part of the regulatory requirements to monitor and control the spread of Johne's disease.

How to fill out APHIS-Approved Laboratories Johne's Disease Serologic Tests?

To fill out APHIS-Approved Laboratories Johne's Disease Serologic Tests, individuals must provide information such as the identification of the animal being tested, the laboratory conducting the test, the date of the test, and the specific type of serologic test performed. It is essential to follow any specific guidelines provided by APHIS regarding the format and required data.

What is the purpose of APHIS-Approved Laboratories Johne's Disease Serologic Tests?

The purpose of APHIS-Approved Laboratories Johne's Disease Serologic Tests is to provide reliable testing methods for identifying infected animals, thereby helping to manage and prevent the spread of Johne's disease in livestock populations. This supports herd health and food safety.

What information must be reported on APHIS-Approved Laboratories Johne's Disease Serologic Tests?

The information that must be reported on APHIS-Approved Laboratories Johne's Disease Serologic Tests includes the name and address of the owner of the animal, the identification number of the animal, the date of testing, the test result, and any additional details required by APHIS regulations to ensure accuracy and accountability.