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Questions & Answers Aug. 2011 DOH 331181 Revised Coliform Bacteria and Drinking Water Public water systems must deliver safe and reliable drinking water to their customers 24 hours a day, 365 days

How to fill out koliformni bacterii

How to fill out koliformni bacterii:

1. Start by preparing a sterile Petri dish and agar medium specifically formulated for growing coliform bacteria.
2. Using a sterile inoculation loop or swab, collect a sample from the desired source where coliform bacteria may be present. This could be a water source, food sample, or environmental surface.
3. Transfer the collected sample onto the surface of the agar medium in the Petri dish. Gently spread the sample to ensure even distribution across the agar surface.
4. Close the Petri dish using its lid and secure it with adhesive tape to prevent contamination from the surroundings.
5. Incubate the Petri dish at the appropriate temperature for coliform bacteria growth, typically around 35-37°C (95-98.6°F), for a specified period of time, usually 18-24 hours.
6. After the incubation period, carefully observe and examine the Petri dish for the presence of characteristic coliform bacterial colonies. These colonies usually appear as small, round, and gram-negative colonies with a metallic green sheen.
7. Count the number of coliform bacterial colonies and record the results. This can help determine the level of coliform bacteria present in the sampled source.
8. Additionally, you may perform further confirmatory tests, such as the use of selective media like MacConkey agar or performing biochemical tests, to identify the specific type of coliform bacteria, like Escherichia coli.
9. Properly dispose of the agar plate and any bacterial waste generated during the process to prevent the spread of any potential pathogens.

Who needs koliformni bacterii:

1. Environmental researchers and scientists studying water quality may need to identify and quantify coliform bacteria in various water sources to assess the level of contamination.
2. Public health officials and regulatory bodies may require testing for coliform bacteria in food samples to ensure safety and compliance with food hygiene standards.
3. Industries involved in food production, such as dairy or meat processing plants, may need to monitor coliform bacteria levels to maintain product quality and safety.
4. Agricultural professionals and farmers may utilize coliform bacteria testing to evaluate the microbial quality of irrigation water, manure, or other agricultural inputs.
5. Medical laboratories and healthcare facilities may perform coliform bacteria testing as part of screening for potential infections or monitoring the efficacy of disinfection procedures in medical equipment sterilization.
6. Water treatment facilities and wastewater treatment plants may need to monitor coliform bacteria levels in their water sources and treatment processes to ensure public health and compliance with regulations.
7. Researchers studying microbial ecology and biodiversity may use coliform bacteria as indicators for various environmental studies, such as studying the impact of pollution on ecosystems.
8. Educational institutions may include coliform bacteria testing as part of microbiology or environmental science curricula to provide hands-on learning experiences for students.

For pdfFiller’s FAQs

What is koliformni bacterii?

Koliformni bacterii are a specific group of bacteria that are commonly used as indicators of fecal contamination in water.

Who is required to file koliformni bacterii?

Any entity or individual responsible for monitoring water quality may be required to file koliformni bacterii.

How to fill out koliformni bacterii?

To fill out koliformni bacterii, one must collect water samples, perform laboratory analysis for coliform bacteria, and submit the results to the appropriate regulatory agency.

What is the purpose of koliformni bacterii?

The purpose of koliformni bacterii is to assess the level of fecal contamination in water sources and to ensure the safety of drinking water.

What information must be reported on koliformni bacterii?

The report on koliformni bacterii must include the sampling location, date and time of sampling, method of analysis, and the results of coliform bacteria presence.

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