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Generating targeting China A. Selecting a target sequence The genomic target sequence should fulfill the following requirements: 20nt long followed by a 3nt PAM sequence: EGG begin with a G to optimize
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Browse through the available protocols and select the one that best fits your research needs. Click on the protocol to open it.
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Read through the protocol carefully to understand the steps and requirements involved. Pay attention to any specific instructions or materials listed.
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Before starting the protocol, make sure you have all the necessary materials and reagents ready. This may include specific enzymes, buffers, or DNA samples.
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Follow the step-by-step instructions provided in the protocol. Make sure to perform each step accurately and in the correct order.
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Take note of any important time points or incubation periods mentioned in the protocol. Set reminders or alarms if necessary.
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Record your observations and results as you follow the protocol. This will help you track your progress and troubleshoot any issues that arise.
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