Get the free Alexa Fluor 488 Phalloidin - Cell Signaling Technology

Confocal immunofluorescent analysis of HeLa cells using Alexa Fluor 488 Phalloidin green. Blue pseudocolor DRAQ5 4084 fluorescent DNA dye. Store at -20 C Alexa Fluor 488 Phalloidin 3 300 assays n Orders n 877-616-CELL 2355 orders cellsignal.com Support n 877-678-TECH 8324 info cellsignal.com Web n www. 21 and 40 CFR 122. 42 Clean Air Act Section 112 Hazardous Air Pollutants HAPs see 40 CFR 61 of 1990. Skin Toxic in contact with skin. Inhalation Harmful by inhalation. Ingestion Harmful if...

How to fill out alexa fluor 488 phalloidin

How to fill out Alexa Fluor 488 phalloidin:

1. Prepare a stock solution of Alexa Fluor 488 phalloidin by dissolving the lyophilized phalloidin powder in the recommended solvent (typically either dimethyl sulfoxide or methanol) to achieve a desired concentration. Follow the instructions provided by the manufacturer for accurate dilution.
2. Carefully handle the Alexa Fluor 488 phalloidin stock solution, as it is light sensitive. Protect it from direct exposure to light by wrapping the container in aluminum foil or storing it in a dark vial.
3. Prior to labeling, ensure that your target sample or specimen is properly fixed and permeabilized according to the recommended protocols. This step may vary depending on the sample type and specific experimental requirements.
4. Dilute the Alexa Fluor 488 phalloidin stock solution into the appropriate buffer or staining solution. The recommended dilution ratio can again be found in the manufacturer's instructions, typically ranging from 1:200 to 1:500. Make sure to mix the solution thoroughly to achieve a homogenous mixture.
5. Apply the diluted Alexa Fluor 488 phalloidin solution to your fixed and permeabilized sample, making sure to cover the entire area of interest. Incubate the sample at the recommended temperature for the suggested duration, according to the manufacturer's instructions.
6. After the incubation period, wash the sample with the appropriate buffer or stain-free solution to remove any unbound Alexa Fluor 488 phalloidin and minimize background noise.
7. Mount the sample on a slide or coverslip using an appropriate mounting medium, which may contain antifade agents to prevent photobleaching and preserve the fluorescence signal.
8. Lastly, visualize and analyze the labeled sample using an appropriate fluorescence microscope or imaging system. Adjust the excitation and emission wavelengths according to the Alexa Fluor 488 spectral properties (typically excitation at around 495 nm and emission at around 519 nm).

Who needs Alexa Fluor 488 phalloidin?

1. Researchers working in cell biology and microscopy studies often require Alexa Fluor 488 phalloidin for visualizing the actin cytoskeleton in fixed and permeabilized cells or tissues. It enables the identification and characterization of actin-rich structures, such as stress fibers, filopodia, lamellipodia, and other actin-based protrusions.
2. Alexa Fluor 488 phalloidin is commonly used in various fields of biology and biomedical research, including developmental biology, cancer biology, neurobiology, immunology, and tissue engineering. Its versatility allows for the visualization and quantification of actin dynamics, cell migration, cellular organization, and other important cellular processes.
3. Additionally, Alexa Fluor 488 phalloidin is utilized by pharmaceutical and biotechnology companies to screen and evaluate potential drug candidates that target the actin cytoskeleton. It provides valuable insights into the effects of these compounds on cell morphology, cell motility, and other actin-related cellular functions.

Overall, anyone studying the actin cytoskeleton and its associated functions in cellular processes can benefit from using Alexa Fluor 488 phalloidin as a reliable and sensitive fluorescent probe.

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What is alexa fluor 488 phalloidin?

Alexa Fluor 488 phalloidin is a high-affinity, F-actin-specific label that is used to visualize actin filaments in cells.

Who is required to file alexa fluor 488 phalloidin?

Researchers and scientists who are using Alexa Fluor 488 phalloidin in their experiments or studies may be required to report its usage.

How to fill out alexa fluor 488 phalloidin?

To fill out information on Alexa Fluor 488 phalloidin, researchers typically provide details on the experiment, the cells or tissue being studied, and the concentration of the phalloidin used.

What is the purpose of alexa fluor 488 phalloidin?

The purpose of Alexa Fluor 488 phalloidin is to specifically label F-actin in cells, allowing for visualization of the actin cytoskeleton and analysis of cell structure.

What information must be reported on alexa fluor 488 phalloidin?

Information typically reported on Alexa Fluor 488 phalloidin includes the experiment details, cell type, concentration used, and any other relevant experimental conditions.