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Modified Hodge Test for Carbapenemase Detection in Enterobacteriaceae Background The Modified Hodge Test (MET) detects carbapenemase production in isolates of Enterobacteriaceae. In the United States,
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How to fill out modified hodge test for
How to fill out the modified hodge test:
01
Obtain the necessary materials: You will need a modified hodge test agar plate, a loop for inoculation, and a pure culture of the organism being tested.
02
Inoculate the modified hodge test agar plate: Using a loop, streak the organism being tested in a straight line from the edge of the agar plate towards the center.
03
Place indicator organisms: Alongside the streak made in step 2, streak an indicator organism known to be susceptible to carbapenem antibiotics.
04
Incubate the plate: Following the manufacturer's guidelines, incubate the plate at the appropriate temperature for the recommended duration.
05
Interpret the results: After incubation, examine the plate for the presence of an inhibition zone around the indicator organism streak. If there is a distortion or "fishtailing" of the growth inhibition zone, it indicates carbapenemase production and a positive result for the modified hodge test.
Who needs the modified hodge test:
01
Healthcare facilities: The modified hodge test is commonly used in healthcare facilities, such as hospitals and clinics, to detect the presence of carbapenemase-producing organisms. This helps in identifying potential outbreaks and guiding appropriate antimicrobial therapy.
02
Infection control teams: Infection control teams within healthcare facilities may utilize the modified hodge test as part of their surveillance and monitoring efforts. It aids in identifying and responding to carbapenem-resistant bacteria, which are a significant concern for healthcare-associated infections.
03
Microbiology laboratories: Microbiology laboratories perform the modified hodge test as part of their routine antimicrobial susceptibility testing for certain bacteria. It provides valuable information for guiding treatment decisions and preventing the spread of antibiotic resistance.
In summary, the modified hodge test is used to detect carbapenemase production in bacteria and is commonly performed by healthcare facilities, infection control teams, and microbiology laboratories. Following the step-by-step procedure outlined above ensures accurate and reliable results.
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What is modified hodge test for?
Modified hodge test is a phenotypic confirmatory test used for detecting carbapenemase-producing Enterobacterales.
Who is required to file modified hodge test for?
Clinical laboratories and healthcare facilities are required to perform and report the results of modified hodge test.
How to fill out modified hodge test for?
Modified hodge test is conducted in a laboratory setting following specific protocols and guidelines.
What is the purpose of modified hodge test for?
The purpose of modified hodge test is to identify the presence of carbapenemase-producing Enterobacterales, which are often resistant to antibiotics.
What information must be reported on modified hodge test for?
The results of the modified hodge test, including whether carbapenemase production was detected or not, must be reported.
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