Get the free h295r cell culture protocol form - epa

The protocol gives a detailed description of the methodology to culture and maintain the H295R human adrenocortical carcinoma cell line ATCC CLR-2128. APPENDIX I - H295R Cell Culture Protocol - Environmental Toxicology Laboratory University of Saskatchewan Saskatoon SK S7N 5B3 Effective Date 26/06/07 SOP 1 Revision 3 Page 1 of 22 Replaces SOP 1 H295R culture Toxicology Centre STANDARD OPERATING PROCEDURE Culturing of the H295R human adrenocortical carcinoma cell line ATCC CLR-2128 Version 3...

How to fill out h295r cell culture protocol

How to fill out the h295r cell culture protocol:

1. Prepare the required cell culture media and supplements according to the specified concentrations and protocols.
2. Use sterile techniques, such as a laminar flow hood, to ensure the cleanliness and purity of the cell culture environment.
3. Seed the h295r cells into appropriate cell culture vessels, such as tissue culture plates or flasks, at the desired density.
4. Allow the cells to adhere and propagate in the incubator under the recommended culture conditions, including temperature, humidity, and CO2 levels.
5. Regularly monitor the cell culture for confluence and viability by microscopic examination and trypan blue staining if necessary.
6. Follow the designated schedule for media change, usually every 2-3 days, to maintain optimal cell growth and health.
7. Perform any experimental treatments or interventions as required by the specific research objectives.
8. Upon completion of the cell culture protocol, properly dispose of all waste materials and decontaminate the cell culture vessels and equipment.

Who needs the h295r cell culture protocol?

1. Researchers or scientists working with h295r cells in laboratory settings.
2. Biotechnology or pharmaceutical companies developing drugs or studying hormone-related disorders.
3. Academic institutions or research centers investigating adrenal gland function or steroid hormone production.

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What is h295r cell culture protocol?

The h295r cell culture protocol refers to a set of instructions and techniques for growing and maintaining h295r cells in a laboratory setting.

Who is required to file h295r cell culture protocol?

Individuals or organizations conducting research or experiments involving h295r cells are typically required to file the h295r cell culture protocol.

How to fill out h295r cell culture protocol?

To fill out the h295r cell culture protocol, one should provide detailed information about the experimental procedures, culture medium composition, cell culture conditions, and any applicable safety precautions.

What is the purpose of h295r cell culture protocol?

The purpose of the h295r cell culture protocol is to provide a standardized method for culturing and studying h295r cells in order to advance scientific understanding or achieve specific research objectives.

What information must be reported on h295r cell culture protocol?

The h295r cell culture protocol typically requires reporting information such as the source of the cells, culture medium components, cell seeding density, incubation temperature, duration of culture, and any experimental treatments or interventions.