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This document outlines the procedures and criteria for the collection of dried blood spots (DBS) for PCR testing to detect HIV in infants, emphasizing the importance of early diagnosis for treatment.
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How to fill out dbs sample collection for
How to fill out DBS Sample Collection for Infant PCR Test
01
Gather all necessary materials: sample collection kit, lancet, and collection card.
02
Wash your hands thoroughly or use hand sanitizer before handling the materials.
03
Prepare the infant by ensuring they are calm and comfortable.
04
Choose a suitable site on the infant's heel for the blood draw.
05
Use the lancet to make a quick puncture on the heel to obtain a blood droplet.
06
Allow the blood to drop onto the designated circles on the collection card.
07
Ensure there is sufficient blood on each circle, and avoid any contamination.
08
Allow the blood spots to dry completely at room temperature.
09
Place the collection card into the provided envelope and seal it securely.
10
Label the envelope with the required patient information before sending it to the lab.
Who needs DBS Sample Collection for Infant PCR Test?
01
Infants who are suspected of having a genetic disorder or infectious disease.
02
Infants enrolled in newborn screening programs.
03
Infants who require confirmation of a specific condition through PCR testing.
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People Also Ask about
What is DBS in PCR?
• Dried Blood Spots (DBS)/ Plasma are the specimens that can be used to perform. HIV-1 PCR testing.
What is DBS in babies?
A revolutionary method of HIV testing of infants, dried blood spot (DBS) testing, had by then been introduced, significantly cutting the time for diagnosis.
How to prepare DBS?
Transfer 1-2 drops of blood to the center of each of the 5 circles (on both cards) without touching the filer paper directly. Fully saturate the circles. Affix one bar- code labeled sticker to each of the two DBS cards, data collection form, blood tube, and client's medical file.
How do you collect DBS samples?
Using the pipette, transfer 20 µl of blood onto the filter paper (Figure 2E-F). MAKE SURE TO COLLECT ENOUGH BLOOD TO COMPLETELY FILL THE CAPILLARY OF THE PIPETTE. AVOID AIR BUBBLES WHEN COLLECTING BLOOD WITH THE PIPETTE. DELIVER THE ENTIRE AMOUNT OF BLOOD COLLECTED.
How to take a dried blood spot sample?
Dried blood spot As shown above, you use a lance at the side of the finger to puncture the finger. The finger is then squeezed a little and the first drop is wiped away. The next drop of blood is placed on the sampler hole of the collection device. We require two spots so this is repeated.
What is DBS collection?
Dried blood spots (DBS), collected by pricking the finger or heel and dabbing drops of blood on filter paper, can be used as a viable alternative to more expensive and invasive methods of obtaining biological samples. DBS collection is becoming more common and is beneficial in various fields.
What are the three methods of collecting blood samples?
Venous, Arterial, and Capillary Blood Specimens Specimen TypeMethod of Collection Venous Direct puncture of vein by venipuncture; vascular access device Arterial Direct puncture of artery; vascular access device Capillary Dermal puncture of fingertip or heel
How to collect a DBS sample?
Press the lancet toward the finger to puncture the finger, a little off the center of the finger pad (Figure 2C). Discard the lancet after use. Wipe away the first drop of blood with dry, clean cotton wool and apply gentle pressure to the finger (Figure 2D). Discard cotton wool after use.
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What is DBS Sample Collection for Infant PCR Test?
DBS Sample Collection for Infant PCR Test refers to the process of collecting dried blood spots (DBS) from infants for the purpose of performing polymerase chain reaction (PCR) testing to detect specific infections or genetic conditions.
Who is required to file DBS Sample Collection for Infant PCR Test?
Healthcare providers or clinicians responsible for the care of infants are typically required to file the DBS Sample Collection for Infant PCR Test, especially in cases where screening for specific conditions is mandated.
How to fill out DBS Sample Collection for Infant PCR Test?
To fill out the DBS Sample Collection form, provide detailed information such as the infant's name, date of birth, clinical information, and parent or guardian details, ensuring all sections are completed accurately before collection.
What is the purpose of DBS Sample Collection for Infant PCR Test?
The purpose of DBS Sample Collection for Infant PCR Test is to screen infants for certain infectious diseases or genetic disorders early in life, allowing for timely diagnosis and treatment.
What information must be reported on DBS Sample Collection for Infant PCR Test?
The information that must be reported includes the infant's identification details (name, date of birth), date of sample collection, clinical information, reasons for testing, and details of the person collecting the sample.
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