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This document contains the results of a Skin Sensitization Local Lymph Node Assay conducted on a chemical substance to assess its sensitization potential in mice, in accordance with established testing

How to fill out local lymph node assay

How to fill out Local Lymph Node Assay in the Mouse

1. Gather all necessary materials, including mice, lymph node collection tools, and necessary assays.
2. Select an appropriate mouse strain, usually C57BL/6 or BALB/c.
3. Prepare the test compounds to be administered to the mice, ensuring accurate dosing.
4. Inject the selected test compounds into the base of the tail of the mice or via other approved routes.
5. Allow an appropriate incubation period for the test compounds to take effect, typically 24-72 hours.
6. Euthanize the mice according to ethical guidelines and collect the draining lymph nodes.
7. Dissect the lymph nodes carefully and prepare them for analysis, typically by homogenization or evaluation through flow cytometry.
8. Analyze the data collected from the assays to determine the immune response.

Who needs Local Lymph Node Assay in the Mouse?

1. Researchers conducting immunotoxicity studies to assess the safety of chemicals.
2. Pharmaceutical companies developing new drugs and vaccines.
3. Regulatory agencies evaluating potential allergens or skin sensitizers.
4. Academic institutions studying immune responses in mice.

People Also Ask about

What are local lymph nodes?

Lymph nodes are located in many parts of the body, including the neck, armpit, chest, abdomen (belly), and groin. They contain immune cells that can help fight infection by attacking and destroying germs that are carried in through the lymph fluid. There are hundreds of lymph nodes throughout the body.

How many lymph nodes are there in total?

Lymph nodes are found at the convergence of major blood vessels, and an adult will have approximately 800 nodes commonly sited in the neck, axilla, thorax, abdomen, and groin. These filter incoming lymph and play a role in infection as well as in malignancy.

How many mammary glands do mice have?

In mice, there are five pairs of mammary glands located along the ventral milk line between the neck and perianal area. Male mice lack s because the mammary tissue regresses in males during embryonic development.

Are there multiple lymph nodes?

There are hundreds of lymph nodes throughout the body. Each lymph node filters the fluid and substances picked up by the vessels that lead to it.

How many lymph nodes are in mice?

There are 13 lymphocenters (mandibular, deep cervical, axillary, mediastinal, bronchial, coeliac, cranial mesenteric, caudal mesenteric, iliac, lumbar, sacral, subiliac and popliteal) which are comprised of 16 groups of lymph nodes.

What is the local lymph node assay for skin sensitization?

The murine local lymph node assay (LLNA) is an in vivo test for skin sensitisation. LLNA has largely superseded the guinea pig maximisation test and the Buehler test. It is considered more scientific and less cruel (lower number of animals; less suffering) and has found broad scientific and regulatory acceptance.

How many lymph nodes do mice have?

Murine lymph nodes are intensively studied but often assigned incorrectly in scientific papers. In BALB/cAnNCrl mice, we characterized a total of 22 different lymph nodes.

For pdfFiller’s FAQs

What is Local Lymph Node Assay in the Mouse?

The Local Lymph Node Assay (LLNA) in the Mouse is a testing method used to evaluate the potential of a chemical substance to cause allergic skin reactions. It assesses the immune response in the local lymph nodes of the mouse following topical exposure to the test substance.

Who is required to file Local Lymph Node Assay in the Mouse?

Manufacturers or companies that are developing or marketing chemical substances, especially those that may have skin sensitization potential, are required to file the Local Lymph Node Assay in the Mouse as part of regulatory compliance.

How to fill out Local Lymph Node Assay in the Mouse?

To fill out the LLNA report, one must document the test substance, dose levels, test conditions, results of the assay, observations on lymph node enlargement, and any other pertinent data, ensuring adherence to regulatory guidelines.

What is the purpose of Local Lymph Node Assay in the Mouse?

The purpose of the Local Lymph Node Assay in the Mouse is to determine the sensitizing potential of chemical substances and to provide data that can be used to assess the risk of allergic reactions in humans.

What information must be reported on Local Lymph Node Assay in the Mouse?

The information that must be reported includes the identification of the test substance, the method of application, the concentration used, the number of animals tested, the results of lymph node weight measurements, and any observed signs of systemic toxicity.