Get the free 384-WELL PCR PLATE RUN FORM - mssm

This form is used to submit information for each plate being run in a qPCR facility, including user details, plate specifications, and protocol options.

How to fill out 384-well pcr plate run

How to fill out 384-WELL PCR PLATE RUN FORM

1. Obtain the 384-WELL PCR PLATE RUN FORM from your laboratory’s standard operating procedures or designated resource.
2. Fill in the date of the run at the top of the form.
3. Enter your name and the names of any collaborators involved in the experiment.
4. Specify the project or experiment name to which this run pertains.
5. List the sample IDs in the provided columns or sections, ensuring that each sample corresponds to its designated well in the plate.
6. Include relevant information such as template concentration, primer sequences, and any other necessary details for each sample.
7. Indicate the PCR setup details, including the type of PCR machine used, thermal cycling conditions, and any reagents added.
8. Note any controls that will be run alongside the samples, including positive and negative controls.
9. Review the form for accuracy and completeness.
10. Submit the completed form to the designated laboratory personnel or database according to your lab’s protocol.

Who needs 384-WELL PCR PLATE RUN FORM?

1. Researchers conducting experiments that involve PCR amplification.
2. Laboratory technicians preparing and organizing PCR runs.
3. Quality assurance personnel who require documentation of PCR processes.
4. Anyone involved in the planning and execution of experiments utilizing 384-well PCR plates.

People Also Ask about

How many cells to seed in a 384 well plate?

From these observations, the optimal growth conditions for PC-3 cells on the 384 well plate is recommended to be between 1000-5000 cells per well for up to four days culture.

How many rows are in a 384 well plate?

For those who do not know, a 96 well plate has eight alphabetically labeled rows (A, B, C, D, E, F, G, H) and 12 numerically labeled columns (1:12) and a 384 well plate has 16 alphabetically labeled rows (A:P) and 24 numerically labeled columns (1:24).

What is 384 well spacing?

Each well holds nanoliters to milliliters of liquid. 384-well microplates quadruple well density with a well-to-well spacing of 4.5 mm and a volume of 120 µl.

What is the maximum volume of a 384 well PCR plate?

Product Options 384-Well PCR PlatesReaction VolumeAvailable Colors Hard-Shell 384-Well PCR Plates 1–30 µl (50 µl maximum) Clear, white, pink, blue, green, or yellow Hard-Shell 480 384-Well PCR Plates 1–30 µl (50 µl maximum) Clear or white Microseal 384-Well PCR Plates 5–25 µl (40 µl maximum) Clear, white, or black

What is the capacity of the 384 well plate well?

384 well flat bottom, non-treated microtiter plate with an optimal working volume of 15-145 μL; maximum well capacity of 152 μL.

How many rows and columns are in a 384 well plate?

HardShell PCR Plates Well format96-well384-well rows 8 16 columns 12 24 Well volume 200 µL 50 µL Recommended working volume 150 µL-160 µL 37.5 µL-40 µL7 more rows

What is the recommended volume of a 384 well plate?

384 well flat bottom, non-treated microtiter plate with an optimal working volume of 15-145 μL; maximum well capacity of 152 μL.

What is a 384 well plate used for?

Choices of different 384-well plates are available below for use in high-throughput research applications like PCR, biochemical and cell-based assays, ELISAs, screening, and more. Designed to be handled by automation equipment, 384-well plates share similar dimensions with the standard 96-well plate.

For pdfFiller’s FAQs

What is 384-WELL PCR PLATE RUN FORM?

The 384-WELL PCR PLATE RUN FORM is a documentation tool used to track and record the details of experiments conducted using a 384-well PCR plate format, often utilized in high-throughput screening and analysis.

Who is required to file 384-WELL PCR PLATE RUN FORM?

Researchers, lab technicians, or any personnel conducting experiments involving 384-well PCR plates are typically required to file the 384-WELL PCR PLATE RUN FORM to ensure proper tracking and compliance.

How to fill out 384-WELL PCR PLATE RUN FORM?

To fill out the 384-WELL PCR PLATE RUN FORM, one must provide details such as the experiment date, sample identifiers, PCR conditions, reagent volumes, and results from the run, ensuring all relevant fields are completed accurately.

What is the purpose of 384-WELL PCR PLATE RUN FORM?

The purpose of the 384-WELL PCR PLATE RUN FORM is to maintain a comprehensive record of PCR runs conducted in a 384-well format, facilitating reproducibility, data integrity, and compliance with laboratory protocols.

What information must be reported on 384-WELL PCR PLATE RUN FORM?

The information that must be reported includes the experiment title, date of the run, sample names, assay types, PCR conditions (such as temperatures and times), reagent details, and observations or results obtained from the experiment.