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In this video I'm going to show you how to do a forward and reverse AGO RH type first thing you're going to need is for your forward you're going to your aunt's A or a B and your aunt Die you're also going to need for your reverse typing you're going to need your agent red cells a1 and B, and then you're also going to need some tubes and some plasma and some red cells now these are red cells that have been spun, so I need to make a suspension, and I'm shooting for approximately three percent, and I'm using physiological saline for this if you're not exactly sure what three percent should look like you can use your reagent red cells to make a comparison just give it a good mix and the reagent red cells should be about two to four percent so if they look the same you're pretty close alright once you have all your stuff together you can go ahead, and you can start to label all your tubes and then from there we're start with the four we're going to add one drop all of these reagents so one drop of each of antis era in two are the appropriate tubes that was the then you add the anti-v and then lastly for the forward is the anti D antis area remember just one drop for each of these and then moving on to the reverse portion you're going to use the range of red cells and once again it's a one drop of each one of these in the corresponding tube and just make sure you mix them up a little because they tend to settle the red cells at the bottom of these reagents as you'll see here with this B, so you see the cells and the bottoms just give it a little mix until the cells are gone off the bottom there and then just one drop then from here we're going to go ahead, and I guess you could start either way with these, but I go ahead and sort with the plasma and for Pay we have two drops patient plasma to each one of the reverse tubes or the reagent red cells and then with the patient red blood cells it's just one drop in each of the antis arrow tubes, and after you've added your patient specimens to each of the tubes you go ahead and send refused this, and it will move on to the agglutination viewer and what you're basically trying to do here is to gently decant the sell button and just remove it from the bottom of that tube you can see there we're getting a zero reaction all red blood cells for the sell button are completely going off there's no of Nations again with the B this is the forward that we're doing here once again we're not getting any agglutination when we decant the sell button we suspend it you see there's zero there sorry this is a little awkward to see and then moving on to the ante D now this is the first one we're going to get a positive with you'll see that this has a four plus perfect cell buttons glutton action right there and from that forward you know this is an operation then with the reverse both the a1 and the B reagent red cells are both for positives you can see that single cell button and that's basically if I'm just want to make...
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