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ABO BLOOD GROUPING Tube Method I. Principle The ABO system is the most important blood group antigen system. Individuals who lack the A and/or B antigens on their red blood cells generally have naturally occurring antibodies in their plasma that are directed against the missing antigens. For this reason ABO grouping should include both forward cell and reverse plasma or serum procedures. Proper determination of the ABO group is necessary when selecting red blood cell-containing products for...
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How to fill out the tube method of blood?

01
First, gather all the necessary materials for collecting a blood sample. This includes a sterile needle, tubes with appropriate additives for different tests, alcohol swabs, and bandages.
02
Prepare the patient for the blood collection procedure. Ensure they are in a seated or lying position, with their arm extended and secured properly. Use an alcohol swab to clean the area where the needle will be inserted.
03
Choose an appropriate tube for the required tests. Each tube is color-coded based on the additive it contains. Gently invert the tube a few times to mix the additive before collecting blood.
04
Use the needle to puncture the patient's vein at a suitable angle. This should be done swiftly and smoothly to minimize discomfort. Once the blood starts flowing, it will enter the tube through the needle.
05
Fill the tube with the required amount of blood according to the specific test requirements. The volume needed may vary based on the test being conducted. Ensure that the tube is properly sealed and labeled with the patient's information.
06
After the collection, apply a sterile bandage to the puncture site and instruct the patient to apply pressure to stop any bleeding. Properly dispose of the used needle and other sharps in a designated sharps container.

Who needs the tube method of blood?

01
Individuals undergoing diagnostic tests: The tube method of blood collection is commonly used in laboratories and clinics to collect blood samples for diagnostic purposes. It allows healthcare professionals to assess various parameters such as blood cell counts, glucose levels, lipid profiles, and hormonal imbalances.
02
Healthcare workers and phlebotomists: Proper knowledge and training in the tube method of blood collection are essential for healthcare workers and phlebotomists who perform blood collection procedures. Having the necessary skills ensures accurate and efficient sample collection, reducing the risk of errors and ensuring patient safety.
03
Researchers and scientists: The tube method of blood collection is also utilized by researchers and scientists conducting studies in fields such as genetics, immunology, or pathology. It provides them with access to blood samples for experimental analysis and investigations.
Remember, always consult with a healthcare professional or follow institutional guidelines for specific instructions on collecting blood samples using the tube method.

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In this video I'm going to show you how to do a forward and reverse AGO RH type first thing you're going to need is for your forward you're going to your aunt's A or a B and your aunt Die you're also going to need for your reverse typing you're going to need your agent red cells a1 and B, and then you're also going to need some tubes and some plasma and some red cells now these are red cells that have been spun, so I need to make a suspension, and I'm shooting for approximately three percent, and I'm using physiological saline for this if you're not exactly sure what three percent should look like you can use your reagent red cells to make a comparison just give it a good mix and the reagent red cells should be about two to four percent so if they look the same you're pretty close alright once you have all your stuff together you can go ahead, and you can start to label all your tubes and then from there we're start with the four we're going to add one drop all of these reagents so one drop of each of antis era in two are the appropriate tubes that was the then you add the anti-v and then lastly for the forward is the anti D antis area remember just one drop for each of these and then moving on to the reverse portion you're going to use the range of red cells and once again it's a one drop of each one of these in the corresponding tube and just make sure you mix them up a little because they tend to settle the red cells at the bottom of these reagents as you'll see here with this B, so you see the cells and the bottoms just give it a little mix until the cells are gone off the bottom there and then just one drop then from here we're going to go ahead, and I guess you could start either way with these, but I go ahead and sort with the plasma and for Pay we have two drops patient plasma to each one of the reverse tubes or the reagent red cells and then with the patient red blood cells it's just one drop in each of the antis arrow tubes, and after you've added your patient specimens to each of the tubes you go ahead and send refused this, and it will move on to the agglutination viewer and what you're basically trying to do here is to gently decant the sell button and just remove it from the bottom of that tube you can see there we're getting a zero reaction all red blood cells for the sell button are completely going off there's no of Nations again with the B this is the forward that we're doing here once again we're not getting any agglutination when we decant the sell button we suspend it you see there's zero there sorry this is a little awkward to see and then moving on to the ante D now this is the first one we're going to get a positive with you'll see that this has a four plus perfect cell buttons glutton action right there and from that forward you know this is an operation then with the reverse both the a1 and the B reagent red cells are both for positives you can see that single cell button and that's basically if I'm just want to make...

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The determination of blood group is a laboratory process used to identify an individual's blood type based on the presence or absence of specific antigens on the surface of red blood cells.
Typically, individuals who are undergoing medical procedures that require blood transfusions, organ transplants, or prenatal care may be required to file a determination of blood group.
To fill out a determination of blood group, a healthcare professional will usually collect a blood sample, perform the necessary tests to identify the blood type, and then record the results on a designated form.
The purpose of determining blood group is to ensure safe blood transfusions, to provide proper medical treatment, and to manage pregnancy-related issues such as Rh incompatibility.
The information that must be reported includes the individual's blood type (A, B, AB, or O), Rhesus factor (positive or negative), and any additional relevant notes from the testing process.
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