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This memorandum outlines the requirements for obtaining informed consent from patients before conducting HIV testing, in accordance with North Carolina state law and UNC Health Care System policy.
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How to fill out Specimens Collected for Human Immunodeficiency Virus (HIV) Antibody Testing & HIV p24 Antigen Testing

01
Gather all necessary supplies, including specimen containers, labels, and requisition forms.
02
Ensure all equipment is clean and has not been previously used for other specimens.
03
Identify the patient and confirm their consent for testing.
04
Collect the blood sample by performing venipuncture or fingerstick as appropriate.
05
Immediately transfer the collected blood into the designated specimen container.
06
Label the specimen container with the patient's information, date, and type of test.
07
Complete the requisition form, ensuring all required fields are filled out accurately.
08
Store the specimen as per the laboratory guidelines until it is transported.
09
Transport the specimen to the laboratory promptly, ensuring it remains at the appropriate temperature.

Who needs Specimens Collected for Human Immunodeficiency Virus (HIV) Antibody Testing & HIV p24 Antigen Testing?

01
Individuals who are at risk of HIV infection.
02
Patients showing symptoms suggestive of HIV.
03
Pregnant women as part of routine prenatal screening.
04
Healthcare workers exposed to potential HIV exposures.
05
Individuals undergoing high-risk behaviors such as injection drug use or unprotected sex.
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A positive HIV antibody test means that the body has been exposed to HIV (and the body has produced antibodies in response to this exposure). A person with a positive HIV test will need to have further testing done to confirm this diagnosis.
A rapid antigen/antibody test done with blood from a finger stick can usually detect HIV 18 to 90 days after exposure. An antigen/antibody lab test using blood from a vein can usually detect HIV 18 to 45 days after exposure. A NAT can usually detect HIV 10 to 33 days after exposure.
An antigen test checks your blood for an HIV antigen, called p24. When you're first infected with HIV, and before your body has a chance to make antibodies to the virus, your blood has a high level of p24. The p24 antigen test is accurate 11 days to 1 month after getting infected.
An HIV test checks a sample of your blood, saliva (spit), or (pee) to see whether you are infected with HIV (human immunodeficiency virus). HIV is a virus that destroys certain cells in your immune system. These cells protect your body against diseases from germs, such as bacteria, viruses, and fungi.
Blood (serum, plasma, DBS) is the preferred specimen for testing because it has a higher concentration of HIV antibodies than oral fluid. It also allows for additional testing, including for syphilis, hepatitis B and hepatitis C, and for special studies of HIV type and subtype, and ARV resistance.
Antigen/antibody tests can detect both HIV antibodies and HIV antigens (a protein of the virus) in the blood and are the most used HIV tests. After HIV exposure, antigens will show up in the blood of a recently exposed person sooner than antibodies.
Depending on the test, you'll provide a blood sample (from a vein or a finger ) or an oral fluid sample that can be tested for HIV. Oral fluid contains cells from your mouth. It's different from saliva. Nucleic acid (NAT) test: The NAT test can detect HIV RNA, the genetic material in HIV.
The HIV p24 antigen is the most abundant HIV protein and is essential for assembly of the capsid that encases HIV genetic material. Detection of the HIV p24 antigen is used clinically to diagnose early HIV infection, during which HIV-specific antibodies are not yet detectable.

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Specimens collected for HIV Antibody Testing and HIV p24 Antigen Testing include blood samples, typically serum or plasma, which are used to detect the presence of antibodies against HIV or the p24 antigen, an early marker of HIV infection.
Healthcare providers, laboratories, and testing facilities are required to file specimens collected for HIV Antibody Testing and HIV p24 Antigen Testing to ensure accurate tracking and reporting of HIV cases.
To fill out specimens collected for HIV testing, specify the patient's demographic information, the type of test conducted, the date of collection, and any identifying information required by the testing facility or laboratory.
The purpose of these specimens is to diagnose HIV infection, monitor the progression of the virus, and evaluate the effectiveness of antiretroviral treatment.
Information that must be reported includes the patient's name, date of birth, specimen type, test results, date of collection, and any relevant medical history that may impact testing and interpretation.
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