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This document outlines a funding request to acquire a multiphoton capable/single photon confocal microscope for the Center for Biologic Imaging at the University of Pittsburgh, highlighting the justifications
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How to fill out request for confocalmultiphoton equipment
How to fill out Request for Confocal/multiphoton equipment
01
Start by obtaining the Request for Confocal/multiphoton equipment form from the designated source.
02
Fill in your personal information, including name, contact details, and affiliation.
03
Specify the type of confocal or multiphoton equipment you require.
04
Describe the purpose of the equipment usage and your research objectives.
05
Indicate the duration for which you will need the equipment.
06
Provide any additional information or specifications related to the equipment requirements.
07
Include any necessary approvals or signatures from your supervisor or department head.
08
Review the completed form for accuracy and completeness.
09
Submit the form to the appropriate department or committee for review.
Who needs Request for Confocal/multiphoton equipment?
01
Researchers involved in biological or medical imaging projects.
02
Graduate students conducting experiments requiring advanced microscopy.
03
Laboratories seeking to enhance their imaging capabilities for specific research.
04
Facility managers responsible for overseeing usage of confocal/multiphoton instruments.
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People Also Ask about
What is multiphoton microscopy used for?
Multiphoton microscopy is a powerful tool for visualizing cellular and subcellular events within living tissue with its inherent “optical sectioning” capability, deeper and minimal phototoxicity and photobleaching. Multiphoton microscopy can capture whole organisms or embryos on a large scale.
What is the difference between confocal and brightfield microscopy?
Depth of field: Widefield microscopy captures images of the entire thickness of the sample, including out-of-focus structures, whereas confocal microscopy only captures images of the in-focus plane. Resolution: Confocal microscopy produces sharper images with higher resolution than widefield microscopy.
What is the difference between confocal and TEM microscopy?
Confocal Microscopy: Has a resolution limit of approximately 200 nanometers due to the diffraction limit of light. Electron Microscopy: Can achieve nanometer to sub-nanometer resolution, allowing for the visualization of subcellular structures and even individual molecules in some cases.
What is the difference between confocal and normal microscopy?
Instead of using an incoherent tungsten or mercury lamp source like conventional microscopes, confocal microscopes use a laser to illuminate the sample. The system then collects images at planes at various depths in the sample, known as optical sections, by scanning the laser through different focus positions.
What is the purpose of a confocal microscope?
The primary functions of a confocal microscope are to produce a point source of light and reject out-of-focus light, which provides the ability to image deep into tissues with high resolution, and optical sectioning for 3D reconstructions of imaged samples.
How much does confocal microscopy cost?
Prices for a decent but very basic confocal microscope start at around 100,000 US dollars, while list prices for high-end confocal systems sometimes reach a million dollars.
What is the difference between confocal and multiphoton microscopy?
The wavelengths used in multi-photon microscopy have about half the energy of excitation beams used in confocal microscopy, tending to the red to near-infrared range, and one laser can be used to excite fluorophores with different emission wavelengths simultaneously.
What is multiphoton microscopy used for?
Multiphoton microscopy is a powerful tool for visualizing cellular and subcellular events within living tissue with its inherent “optical sectioning” capability, deeper and minimal phototoxicity and photobleaching. Multiphoton microscopy can capture whole organisms or embryos on a large scale.
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What is Request for Confocal/multiphoton equipment?
A Request for Confocal/multiphoton equipment is a formal submission made to acquire or access specialized imaging equipment used in biological and materials research.
Who is required to file Request for Confocal/multiphoton equipment?
Typically, researchers, scientists, or students who require access to confocal or multiphoton microscopy equipment for their projects or experiments are required to file this request.
How to fill out Request for Confocal/multiphoton equipment?
To fill out the request, applicants need to provide their name, affiliation, project details, specific equipment needs, and desired timeframes for usage, along with any relevant funding or approval information.
What is the purpose of Request for Confocal/multiphoton equipment?
The purpose of the request is to ensure proper allocation, scheduling, and usage of confocal/multiphoton equipment, maintain oversight, and facilitate access for scientific research.
What information must be reported on Request for Confocal/multiphoton equipment?
The request must report the user's contact information, project description, specific experimental requirements, scheduled dates and times for usage, and any necessary safety or compliance information.
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