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Searchable High-Resolution 2D Gel Proteome of the Human Colon Crypt Bhavinkumar B. Patel Xin-Ming Li Maketa P. Dixon Elena L. Blagoi Steven H. Seeholzer Yibai Chen C. Glenn Miller Yin A. He Mazell Tetruashvily Anam H.
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How to fill out Searchable High-Resolution 2D Gel Proteome of the Human Colon Crypt
01
Obtain the appropriate sample of human colon tissue.
02
Prepare the tissue samples by homogenizing and centrifuging to extract proteins.
03
Use two-dimensional gel electrophoresis (2DE) to separate proteins by isoelectric point and molecular weight.
04
Stain the gel with a suitable dye (e.g., Coomassie Brilliant Blue) to visualize protein spots.
05
Use a gel imaging system to capture high-resolution images of the stained gel.
06
Analyze the gel images using software to identify and catalog the proteins based on their location.
07
Create a searchable database to store the data on protein characteristics and their corresponding locations in the gel.
Who needs Searchable High-Resolution 2D Gel Proteome of the Human Colon Crypt?
01
Researchers in the field of proteomics.
02
Biologists studying human colon health and diseases.
03
Clinicians aiming to understand protein variations in colon cancer.
04
Pharmaceutical companies developing targeted therapies for gastrointestinal disorders.
05
Educational institutions teaching advanced biology and proteomics.
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What is Searchable High-Resolution 2D Gel Proteome of the Human Colon Crypt?
The Searchable High-Resolution 2D Gel Proteome of the Human Colon Crypt is a detailed representation of the protein composition found in the colon crypts of humans. It provides a high-resolution mapping of proteins, facilitating the study of their functions and interactions.
Who is required to file Searchable High-Resolution 2D Gel Proteome of the Human Colon Crypt?
Researchers and scientists who conduct studies related to human colon biology, proteomics, or related fields are required to file data pertaining to the Searchable High-Resolution 2D Gel Proteome of the Human Colon Crypt.
How to fill out Searchable High-Resolution 2D Gel Proteome of the Human Colon Crypt?
To fill out the Searchable High-Resolution 2D Gel Proteome of the Human Colon Crypt, one must collect data on protein samples, document experimental methods, and include results in a standardized format that includes relevant metadata such as sample origin, experimental conditions, and analysis techniques.
What is the purpose of Searchable High-Resolution 2D Gel Proteome of the Human Colon Crypt?
The purpose of the Searchable High-Resolution 2D Gel Proteome of the Human Colon Crypt is to enhance our understanding of the protein landscape of the human colon, aiding in research related to digestive health, disease mechanisms, and potential therapeutic targets.
What information must be reported on Searchable High-Resolution 2D Gel Proteome of the Human Colon Crypt?
Information that must be reported includes the types of proteins identified, their concentrations, the experimental conditions used for separation and analysis, and any relevant metadata that contextualizes the findings.
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