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Applause Music this video will demonstrate how to collect a buccal swab for the detection of mumps virus buccal swabs are the preferred sample for confirmation of mumps infection since viral detection assays such as rapper are more sensitive and specific than geologic assays to detect IGM ideally a buccal swab should be collected one to three days after symptom onset but may be collected up to five days after symptom onset the buccal cavity is located near the upper rear molars between the cheek and the teeth when you look inside the mouth you may be able to see a raised area of tissue that represents the Stepson's duct which is a small tube that connects the parotid salivary gland to the oral cavity saliva that is produced in the parotid gland flows through the Stepson's duct into the mouth the parotid gland is located in front of and just below the earlobe at the angle of the jaw to begin collecting a buccal swab the parotid gland is massaged firmly for 30 seconds message is most often painless except in cases where the patient has parotitis or inflammation of the parotid gland and in that case there may be some mild discomfort massaging the parotid is important to obtain the best sample for processing the supplies needed to collect the buccal swab include a flocked swab preferably synthetic and a tube of viral transport medium or ATM and eye nose mouth protection a tongue blade and penlight may be used to locate the Stepson's duct explain to the seated patient the importance of collecting a buccal swab to test for the mumps virus instruct the patient to open her mouth locate the Stepson's duct on both sides using a tongue blade instruct the patient to close her mouth press and firmly massage the parotid gland for 30 seconds put on gloves sweep the swab between the cheek and gum near the upper molar to the lower molar area across the duct for 10 to 15 seconds turning the swab while sweeping sweet both sides uncap the tube insert the swab in at least two milliliters of viral transport medium break off the handle of the swab discard the handle of the swab cap the tube leaving the tip of the swab in the tube label the sample is shipped within 24 hours of collection refrigerated at 4 degrees Celsius which is 39 degrees Fahrenheit ship the sample to the laboratory on cold packs if the sample is shipped more than 24 hours after collection freeze at minus 70 degrees Celsius which is minus 94 degrees Fahrenheit ship the sample to the laboratory on dry ice for more information on collecting and shipping specimens visit this CDC webpage
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