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Get the free Biosafety Protocol Form for Use of rDNA and Infectious Agents - ehs illinoisstate

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This document is a protocol form required for research involving recombinant DNA and infectious agents at Illinois State University, detailing principal investigator information, experiment protocols,
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How to fill out Biosafety Protocol Form for Use of rDNA and Infectious Agents

01
Obtain the Biosafety Protocol Form from your institution's biosafety office or website.
02
Provide general information including the title of the project, principal investigator's name, and contact information.
03
Describe the purpose and objectives of the research involving rDNA or infectious agents.
04
List the types of rDNA and infectious agents to be used in the research.
05
Detail the facilities and equipment that will be used for the research, including the biosafety level.
06
Outline the procedures for handling, storing, and disposing of rDNA and infectious agents.
07
Describe safety and emergency procedures, including preparedness for exposure or contamination.
08
Include information on training and qualifications of personnel who will be working with rDNA and infectious agents.
09
Sign and date the form, and submit it to the biosafety committee for review.

Who needs Biosafety Protocol Form for Use of rDNA and Infectious Agents?

01
Researchers and laboratories that work with recombinant DNA (rDNA) or infectious agents.
02
Institutions that are conducting experiments involving biohazardous materials.
03
Individuals seeking approval for research projects requiring compliance with biosafety regulations.
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Risk Group 1 organisms do not cause disease in healthy adult humans. Risk Group 2 organisms can cause disease in humans, but the disease is treatable or preventable. Risk Group 3 organisms cause serious disease in humans. Treatments and vaccines for these diseases may exist.
Experiments involving the introduction of recombinant DNA into risk group 2, 3 or 4 agents (mostly BSL2, BSL3, and BSL4 organisms) Experiments in which DNA from risk group 2, 3 or 4 agents (mostly BSL2, BSL3, and BSL4 organisms) is transferred into nonpathogenic prokaryotes or lower eukaryotes.
EHS Biosafety follows the NIH Guidelines categorization of Risk Groups as follows: RG1 – Are not associated with disease in healthy adult humans or animals. RG2 – Are associated with disease which is rarely serious and for which preventative or therapeutics is often available.
Risk Group 2 agents are associated with human or animal diseases that are rarely serious. Effective treatment and preventative measures are available. Examples include: working with human blood, Streptococcus pyogenes, Giardia spp., Microsporum canis, human adenoviruses, Hepatitis virus.
Biosafety Standards & Practices At a minimum, lab practices meeting BSL1 requirements will be used for all recombinant DNA work, even when that work is exempt from the NIH Guidelines. Laboratories will be inspected by EHS prior to the start of any recombinant DNA research at BSL2.
By definition recombinant DNA is chimeric, and the possibility for novel life forms of unknown toxicity or environmental hazard has been a concern since recombinant DNA technology was invented in 1973.

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The Biosafety Protocol Form for Use of rDNA and Infectious Agents is a document that outlines the safety measures and procedures to be followed when conducting research or activities involving recombinant DNA (rDNA) and infectious agents to ensure the safety of researchers, the public, and the environment.
Researchers, scientists, or any institution planning to conduct experiments or work with rDNA or infectious agents are required to file the Biosafety Protocol Form.
To fill out the Biosafety Protocol Form, one must provide detailed information about the research project, including the type of rDNA or infectious agents being used, the objectives of the research, safety measures in place, potential risks, and emergency response protocols.
The purpose of the Biosafety Protocol Form is to ensure that all necessary safety precautions are taken, to assess and mitigate risks associated with the use of rDNA and infectious agents, and to comply with legal and regulatory requirements.
The form must report information such as the identity and classification of the rDNA or infectious agents, the location of the work, personnel involved, the research objectives, safety and containment practices, and any potential environmental or public health impacts.
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