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This document provides guidelines on the use of chicken embryos in research, including notification procedures, methods of disposal, and humane euthanasia protocols in case of inadvertent hatching.
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How to fill out GUIDELINES FOR THE USE OF CHICKEN EMBRYOS

01
Begin by ensuring you have a clear understanding of the purpose of the guidelines.
02
Gather all necessary documentation related to chicken embryos.
03
Review the ethical considerations and regulations pertaining to the use of chicken embryos.
04
Fill out the required forms, providing accurate information about the research objectives.
05
Specify the types of experiments you intend to conduct with the chicken embryos.
06
Detail the methods of care and handling of the embryos during the research.
07
Include any potential risks and safety measures associated with your work.
08
Submit the completed guidelines form to the relevant authority for review.

Who needs GUIDELINES FOR THE USE OF CHICKEN EMBRYOS?

01
Researchers conducting studies involving chicken embryos.
02
Institutions and laboratories working with embryos for educational purposes.
03
Regulatory bodies that oversee the ethical use of animal research.
04
Students engaging in projects that require the use of chicken embryos.
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Because chicken embryos are poikilothermic, embryo metabolic development relies on the incubation temperature, which influences the use of egg nutrients and embryo development. Incubation temperature ranging between 37 and 38°C (typically 37.5–37.8°C) optimizes hatchability.
Chicken embryos have been used as a model for ZIKV infections since 1951 (Taylor, 1952) and today there are multiple research groups using the chicken embryo as a model of Congenital Zika Syndrome (Goodfellow et al., 2016; Willard et al., 2017; Thawani et al., 2018, 2020; Wachholz et al., 2021).
The developing embryo (the chick) feeds on the egg yolk through a series of blood vessels. This is why an egg is so good and nutritious for you to eat.
Other advantages include the fact that the development of chicken embryos is similar to that of mammals; they are cheap, readily available, and have a short incubation time. There is full control of the number of embryos as well as the developmental stage, which allows for rapid research [5].
Under the right conditions, each fertilized egg will grow to be a chick. The yolk and albumen (the white of the egg) provide food for the growing embryo. The albumen also provides the embryo with protection against shock, cushioning the developing embryo in a water sac. The blunt end of the egg contains the air cell.
The chick embryo is a valuable tool in the study of early embryonic development. Its transparency, accessibility and ease of manipulation, make it an ideal tool for studying the formation and patterning of brain, neural tube, somite and heart primordia.
The life cycle of a chicken comprises 5 stages- Egg fertilization, Egg embryo, Chick, Pullet, and Adult. A chicken's life cycle lasts around 21 days, beginning with the hen laying a fertilized egg and ending with the chick hatching.
Chick embryos younger than embryonic day 16 (E16) are assumed to be unable to experience pain. It is recommended that E15 or younger embryos be euthanized by hypothermia, typically by placing the eggs in a -20°C freezer.

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The GUIDELINES FOR THE USE OF CHICKEN EMBRYOS provides a framework for ethical and safe handling of chicken embryos in research and education, ensuring compliance with regulatory standards.
Researchers, educators, and institutions conducting work involving chicken embryos are required to file these guidelines to ensure ethical practices are followed.
To fill out the guidelines, provide all necessary details about the research objectives, methods, and measures in place for ethical treatment and disposal of chicken embryos.
The purpose is to promote the ethical use of chicken embryos in research and educational settings while ensuring compliance with legal and ethical standards.
Information required includes research objectives, methodology, the number of embryos to be used, and protocols for care, handling, and disposal of embryos.
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