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MQP-BIO-DSA-0333 MQP-BIO-DSA-5716 GENERATION OF TRANSGENIC MICE WITH A HEARTSPECIFIC DELETION OF THE JNK-1 & 2 GENES USING MHC-CRE A Major Qualifying Project Report Submitted to the Faculty of the

How to fill out generation of transgenic mice

How to fill out generation of transgenic mice:

1. Obtain a suitable vector carrying the transgene of interest.
2. Prepare the embryos of recipient mice for microinjection of the vector.
3. Perform microinjection of the vector into the pronuclei of the embryos.
4. Transfer the injected embryos into the oviducts of surrogate female mice.
5. Allow the embryos to develop and implant in the surrogate female mice.
6. Monitor the development of the transgenic embryos and look for successful integration of the transgene.
7. After birth, identify the transgenic mice through various methods such as PCR or fluorescence imaging.
8. Begin breeding the transgenic mice with wild-type mice to establish a colony of transgenic mice.

Who needs generation of transgenic mice:

1. Researchers studying the effects of specific genes or genetic modifications in mice.
2. Pharmaceutical companies developing new drugs and therapies that may involve genetic modifications in animals.
3. Scientists investigating the mechanisms of genetic disorders and diseases.
4. Biotech companies working on the development of new technologies and treatments.

Note: This answer is provided for informational purposes only and does not constitute medical or scientific advice.

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What is generation of transgenic mice?

Generation of transgenic mice refers to the process of creating mice with genetic modifications by inserting foreign DNA into their embryos.

Who is required to file generation of transgenic mice?

Researchers or organizations conducting experiments or studies involving transgenic mice are typically required to file generation of transgenic mice.

How to fill out generation of transgenic mice?

The process to fill out generation of transgenic mice forms may vary depending on the specific guidelines of the regulating authority. Generally, it involves providing details about the research objectives, methods of genetic modification, animal welfare considerations, and proposed use of transgenic mice.

What is the purpose of generation of transgenic mice?

The purpose of generating transgenic mice is to study the effects of specific genetic modifications, investigate gene functions, understand disease mechanisms, and develop potential treatments or therapies.

What information must be reported on generation of transgenic mice?

The information that must be reported on generation of transgenic mice includes details about the genetic modifications made, experimental protocols, potential hazards or risks, animal welfare protocols, and the proposed use or application of the transgenic mice.