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Print For life science research only. Not for use in diagnostic procedures. Restriction Endonuclease Hind I ? From Hemophilia influenza Rf Cat. No. 10 779 652 001 Cat. No. 10 779 679 001 Cat. No.
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Prepare the reaction mixture by combining the following components:
01
Restriction endonuclease Hinf I enzyme
02
DNA sample to be digested
03
Buffer specific for Hinf I enzyme
04
Optional: additional reagents (such as BSA or ATP) if recommended by manufacturer
4.1
Gently mix the components to ensure proper dispersion.
4.2
Incubate the reaction mixture at the appropriate temperature for Hinf I enzyme, usually around 37°C. Follow the recommended incubation time provided by the manufacturer, usually between 1 to 2 hours.
4.3
After the incubation period, stop the reaction by heat inactivation or by adding an appropriate stop buffer.
4.4
Proceed with downstream applications, such as agarose gel electrophoresis or DNA purification, as required for your specific experiment.
Who needs restriction endonuclease Hinf I?
01
Researchers working in molecular biology laboratories often use restriction endonuclease Hinf I for DNA manipulation.
02
Scientists studying DNA methylation patterns may use Hinf I to assess DNA methylation status at specific sites.
03
Biotechnologists involved in genetic engineering or DNA cloning techniques may require Hinf I to achieve specific DNA fragment digestions and manipulations.
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