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This document provides information on the Quantikine Mouse EGF Immunoassay, which is a solid-phase ELISA designed for the quantitative determination of mouse Epidermal Growth Factor (EGF) in various

How to fill out mouse egf immunoassay

How to fill out Mouse EGF Immunoassay

1. Gather all necessary materials, including the Mouse EGF Immunoassay kit, samples, and pipettes.
2. Prepare all reagents according to the instructions provided in the kit protocol.
3. Dilute the samples as recommended, if necessary, to ensure they are suitable for the assay.
4. Add the samples and standards to the designated wells on the assay plate.
5. Incubate the plate at the specified temperature for the required amount of time.
6. Wash the wells thoroughly to remove any unbound substances.
7. Add the detection antibody to each well and incubate again as instructed.
8. Wash the wells again to remove unbound detection antibody.
9. Add the substrate solution and incubate until the color develops according to the kit protocol.
10. Stop the reaction by adding the stop solution, and measure the absorbance using a plate reader.

Who needs Mouse EGF Immunoassay?

1. Researchers studying the role of EGF in biological processes.
2. Clinical laboratories in need of assessing EGF levels in biological samples.
3. Pharmaceutical companies developing drugs targeting the EGF pathway.
4. Healthcare professionals monitoring patients with conditions related to EGF.

People Also Ask about

What is the difference between human and mouse EGF?

The biological activity of mouse and human EGF is identical. Both mouse and human EGF bind to the same receptor sites on mouse, human, rat and chick cells. Epidermal Growth Factor (EGF) is one of the best known and characterized growth factors.

What is the difference between human and mouse immune system?

The balance of lymphocytes and neutrophils in adult animals is quite different: human blood is neutrophil rich (50–70% neutrophils, 30–50% lymphocytes) whereas mouse blood has a strong preponderance of lymphocytes (75–90% lymphocytes, 10–25% neutrophils) (10).

What is the difference between human EGF and mouse EGF?

Human, rat and mouse epidermal growth factors (EGF) bind to the same receptor of human placenta, but the binding characteristics differ. The apparent affinity constant (KA) for human EGF is higher (15·109l/mol) than KA for rat EGF (10·109 l/mol). Mouse EGF binds with the lowest KA (5·109 l/mol).

What is human EGF?

Epidermal growth factor (EGF) is a single polypeptide of 53 amino acid residues which is involved in the regulation of cell proliferation. Egf exerts its effects in the target cells by binding to the plasma membrane located EGF receptor. The EGF receptor is a transmembrane protein tyrosine kinase.

Does mouse EGF bind to human EGFR?

Human, rat and mouse epidermal growth factors (EGF) bind to the same receptor on human placenta, but the binding characteristics differ. The apparent affinity constant (KA) for human EGF is higher (15 X 10(9) l/mol) than KA for rat EGF (10 X 10(9) l/mol). Mouse EGF binds with the lowest KA (5 X 10(9) l/mol).

What is EGF on a blood test?

Healthcare providers use estimated glomerular filtration rate (eGFR) to determine if you have kidney disease, and if so, what stage. eGFR measures your kidneys' ability to filter toxins or waste from your blood. A very low number may indicate kidney failure, which requires dialysis or a kidney transplant.

For pdfFiller’s FAQs

What is Mouse EGF Immunoassay?

The Mouse EGF Immunoassay is a laboratory test used to measure the levels of Epidermal Growth Factor (EGF) in mouse biological samples. It typically involves using specific antibodies to detect and quantify EGF concentrations.

Who is required to file Mouse EGF Immunoassay?

Researchers and laboratories conducting studies that involve the assessment of EGF levels in mice are typically required to file the Mouse EGF Immunoassay. This may include academic institutions, biotechnology companies, and pharmaceutical organizations.

How to fill out Mouse EGF Immunoassay?

To fill out the Mouse EGF Immunoassay, users must carefully follow the assay protocol, which includes preparing samples, adding reagents, incubating appropriately, and using a detection method to quantify EGF levels. Detailed instructions are usually provided in the assay kit.

What is the purpose of Mouse EGF Immunoassay?

The purpose of the Mouse EGF Immunoassay is to monitor and evaluate the presence and levels of EGF in mouse samples. This is important for understanding biological processes related to cell growth, development, and response to injury.

What information must be reported on Mouse EGF Immunoassay?

The information that must be reported includes the concentration of EGF in the samples, the type of samples tested (e.g., serum, tissue homogenates), the assay conditions, and any relevant control data to validate the assay results.