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This document provides technical specifications, immunogen details, and application methods for the Mouse Anti-Hsp70 Alkaline Phosphatase Conjugate Monoclonal Antibody, including safety information
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01
Gather the necessary materials, including the Anti-Hsp70:AP Conjugate, pipettes, and sample buffer.
02
Thaw the Anti-Hsp70:AP Conjugate if it is stored frozen, and gently mix by inverting the tube.
03
Dilute the conjugate in an appropriate buffer as per the manufacturer's instructions.
04
Add the diluted Anti-Hsp70:AP Conjugate to your sample wells.
05
Incubate the samples for the recommended time and temperature to allow binding.
06
Following the incubation, wash the wells to remove unbound conjugate.
07
Proceed with the detection method suitable for the enzyme linked to the conjugate.

Who needs Anti-Hsp70:AP Conjugate?

01
Researchers studying stress responses in cells.
02
Clinicians investigating autoimmune diseases.
03
Laboratories conducting research on heat shock proteins.
04
Pharmaceutical companies developing therapies targeting stress response pathways.
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People Also Ask about

Molecular chaperones of the Hsp70 family are conserved proteins that modulate intracellular protein folding. By binding to unfolded or partially folded polypeptides, chaperones prevent misfolding and aggregation and promote folding, translocation, and the assembly and disassembly of multiprotein structures (1, 2).
Hsp70 proteins are central components of the cellular network of molecular chaperones and folding catalysts. They assist a large variety of protein folding processes in the cell by transient association of their substrate binding domain with short hydrophobic peptide segments within their substrate proteins.
HSP70 Antibody detects endogenous levels of total HSP70 protein (HSP70-Hom, HSP70-1) and HSC70. Human, Mouse, Rat, Monkey, Bovine. Goat.
Members of the 70-kDa heat shock protein (hsp70) family play an essential role in protein folding, transport of proteins into different cellular compartments, and regulation of the heat shock response (1, 2). The members of this protein family are highly conserved from bacteria to man (3, 4).
BiP, an HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum (ER), binds newly-synthesized proteins as they are translocated into the ER and maintains them in a state competent for subsequent folding and oligomerization.
Gene expression patterns of the Hsp70 gene in different organs indicated that the liver, spleen, brain and kidney exhibited 5.94, 4.96, 5.29 and 2.63-fold higher mRNA level expressions than the control (Table 1). Liver and brain tissues showed the highest gene expression levels as compared to kidney, spleen and heart.
Hsp70 proteins are central components of the cellular network of molecular chaperones and folding catalysts. They assist a large variety of protein folding processes in the cell by transient association of their substrate binding domain with short hydrophobic peptide segments within their substrate proteins.
Heat shock protein 70 (Hsp70) is a molecular chaperone that is expressed in response to stress. In this role, Hsp70 binds to its protein substrates and stabilize them against denaturation or aggregation until conditions improve.

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Anti-Hsp70:AP Conjugate is an antibody conjugated with an alkaline phosphatase enzyme that is used in various biological assays to detect the presence of the Hsp70 protein.
Researchers and laboratories conducting studies that involve the use of Anti-Hsp70:AP Conjugate for detection or analysis of Hsp70 proteins are required to file the necessary documentation.
To fill out the Anti-Hsp70:AP Conjugate documentation, you should provide details about the experiment, including the quantity used, the source of the conjugate, and any relevant observations or results.
The purpose of Anti-Hsp70:AP Conjugate is to facilitate the detection and quantification of the Hsp70 protein in various samples, aiding in research related to stress responses and protein interactions.
The information that must be reported includes the lot number, expiration date, concentration, usage instructions, and any relevant safety data or hazard information regarding the conjugate.
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