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This document outlines the standard operating procedure for screening urine samples for benzodiazepines using the Immunalysis Benzodiazepines Direct RIA Kit, including background information, methodology,
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How to fill out Screen for Benzodiazepines in Urine

01
Collect a urine sample in a clean, dry container.
02
Ensure the sample is at the appropriate temperature (typically between 90-100 degrees Fahrenheit) to verify its validity.
03
Label the container with the patient's details including name, date, and time of collection.
04
Use a Benzodiazepine test kit according to the manufacturer's instructions.
05
Dip the test strip into the urine sample for the recommended duration (usually a few seconds).
06
Remove the test strip and lay it flat on a clean surface.
07
Wait for the specified time period (usually 5-10 minutes) for results to appear.
08
Interpret the results according to the test kit's guide (positive or negative for Benzodiazepines).
09
Dispose of the test kit and sample in accordance with hazardous waste guidelines.

Who needs Screen for Benzodiazepines in Urine?

01
Patients undergoing treatment for substance use disorders to monitor compliance.
02
Individuals involved in legal cases where drug use requires verification.
03
Patients prescribed Benzodiazepines for anxiety or sleep disorders to ensure proper usage.
04
Employees in safety-sensitive jobs where drug testing is a workplace requirement.
05
Individuals suspected of Benzodiazepine misuse or overdose.
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Typical therapeutic concentrations are 0.1–1.0 mg/L, while toxic effects occur at levels of more than 1.5 mg/L. Fatalities (rare where only one drug is involved) may occur at levels in excess of 5 mg/L.
A typical benzodiazepine test can find benzodiazepines or their break-down products, called metabolites. But this is a very complex test. A positive test result means that the test found the medicine's metabolite in your at the time the sample was taken.
Common OTC medications that cause false positives for benzodiazepines include: Antihistamines. Diphenhydramine (Benadryl) Sleep aids. ZzzQuil (contains diphenhydramine) Cold and flu medications. Many combination cold medicines containing brompheniramine. NSAIDs (less common but possible) Sertraline (Zoloft)
These benzodiazepines include alprazolam (Xanax®), chlordiazepoxide (Librium®), clorazepate (Tranxene®), diazepam (®), halazepam (Paxipam®), lorzepam (Ativan®), oxazepam (Serax®), prazepam (Centrax®), and quazepam (Doral®). Clonazepam (Klonopin®), diazepam, and clorazepate are also used as anticonvulsants.
Reference Range A benzodiazepine (Oxazepam/Lorazepam/Alprazolam metabolite/Clonazepam metabolite/Nordiazepam/Temazepam) result of < 50 is a NEGATIVE result (50 ng/mL is the lower reporting limit for this LCMS confirmation assay). Values reported ≥ to 50 should be interpreted as POSITIVE.
A Drug Screen - Benzodiazepines, Spot blood test is a simple blood draw, similar to other routine blood tests. A healthcare professional will insert a needle into a vein in your arm and collect a small sample of blood, which will be sent to a laboratory for analysis.
Reference Range(s) Temazepam50-1000 ng/mL Diazepam 100-1500 ng/mL Lorazepam 50-240 ng/mL Midazolam 50-600 ng/mL Triazolam 5-20 ng/mL11 more rows
Recommended Cutoff Levels for Initial Tests PCP* 25 ng/ml Benzodiazepines** 100 ng/ml Barbiturates** 300 ng/ml Methadone** 300 ng/ml5 more rows

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The Screen for Benzodiazepines in Urine is a diagnostic test used to detect the presence of benzodiazepines, a class of medications often prescribed for anxiety, insomnia, and other conditions, in a person's urine.
Typically, individuals in safety-sensitive jobs, those applying for certain medical treatments, or patients undergoing substance abuse evaluation may be required to file a Screen for Benzodiazepines in Urine.
To fill out the Screen for Benzodiazepines in Urine, the individual must provide personal information including their name, date of birth, and the date of the sample collection, as well as consent for testing, if required.
The purpose of the Screen for Benzodiazepines in Urine is to identify the use of benzodiazepines, monitor compliance with prescribed treatments, and prevent misuse or abuse of these medications.
The information that must be reported includes the individual's identification details, results of the screening test, the type and concentration of benzodiazepines detected, and the name of the testing facility.
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