Get the free CTAB Plant DNA Extraction USER/BILLING AGREEMENT - plantgenomics iastate

This agreement outlines the billing process and requirements for users of CTAB DNA Extraction services at the Genomic Technologies Facility, Iowa State University. It includes details about fees,

How to fill out ctab plant dna extraction

How to fill out CTAB Plant DNA Extraction USER/BILLING AGREEMENT

1. Obtain a copy of the CTAB Plant DNA Extraction USER/BILLING AGREEMENT template.
2. Read through the agreement carefully to understand its terms and conditions.
3. Fill in your full name and contact information in the designated fields.
4. Provide your organization or institution name, if applicable.
5. Specify the purpose for which the CTAB Plant DNA Extraction will be used.
6. Enter the billing information, including any billing address and payment method details.
7. Sign and date the agreement where indicated.
8. Submit the completed agreement to the designated authority or contact provided.

Who needs CTAB Plant DNA Extraction USER/BILLING AGREEMENT?

1. Researchers conducting DNA extraction from plant samples.
2. Laboratories performing genetic analysis of plant material.
3. Educational institutions involved in plant biology studies.
4. Companies working in agriculture or biotechnology requiring DNA analysis.

People Also Ask about

What are the disadvantages of CTAB method?

The main drawback of this procedure is that it is time-consuming and makes use of toxic chemicals like phenol and chloroform. Moreover, CTAB extracted DNA requires further purification to avoid inhibition of PCR analyzes [32].

Why is CTAB used for plant DNA extraction?

CTAB (also called hexadecyltrimethylammonium bromide) is a cationic detergent that facilitates the separation of polysaccharides during purification while additives, such as polyvinylpyrrolidone, aid in inactivating polyphenols. CTAB based extraction buffers are widely used when purifying DNA from plant tissues.

What is the purpose of chloroform isoamyl alcohol in DNA extraction?

A mixture of phenol:chloroform:isoamyl alcohol (25:24:1) is then added to promote the partitioning of lipids and cellular debris into the organic phase, leaving isolated DNA in the aqueous phase. Following centrifugation, the aqueous phase containing the purified DNA can be transferred to a clean tube for analysis.

What is CTAB used for?

Today, in addition to its use as a topical antiseptic, CTAB has applications in medicine as an apoptosis-promoting anticancer agent, and in protein electrophoresis, DNA extraction buffer systems, and nanoparticle synthesis.

Why is CTAB used in plant DNA extraction?

Plant tissue, which is rich in complex polysaccharides and secondary metabolites, interfere and co-precipitate with DNA; CTAB along with some other chemicals like PVP is used to minimize the effect of these metabolites.

How to prepare 10% CTAB?

10 % (w/v) CTAB - Add 10 g of CTAB to approximately 70 ml of water. Dissolve the detergent by warming the solution to 65 ° C. Adjust the volume to 100 ml. 0.5 M EDTA (pH 8.0) - Add 93.05 g of ethylenediaminetetraacetate.

What is the function of binding buffer in DNA extraction?

The first step in a solid-phase process uses binding buffer that helps to bind DNA to the solid surface by providing the required conditions. These conditions depend on the solid surface and buffer composition, pH, etc., followed by washing with ethanol and buffer.

What is the function of CTAB solution?

CTAB is an essential surfactant in the DNA extraction buffer system that removes membrane lipids and promotes cell lysis. Separation is also successful when the tissue contains high amounts of polysaccharides.

For pdfFiller’s FAQs

What is CTAB Plant DNA Extraction USER/BILLING AGREEMENT?

CTAB Plant DNA Extraction USER/BILLING AGREEMENT is a legal document that outlines the terms and conditions for using a CTAB (Cetyl Trimethyl Ammonium Bromide) method for extracting DNA from plant samples. It typically includes information about the user's responsibilities, billing information, and compliance with any relevant regulations.

Who is required to file CTAB Plant DNA Extraction USER/BILLING AGREEMENT?

Any individual or organization that intends to use the CTAB method for plant DNA extraction and requires billing for services or products associated with this process is required to file the agreement.

How to fill out CTAB Plant DNA Extraction USER/BILLING AGREEMENT?

To fill out the CTAB Plant DNA Extraction USER/BILLING AGREEMENT, users should provide accurate information including their name, contact details, billing information, intended use of the extracted DNA, and any specific requests or additional information as required by the agreement.

What is the purpose of CTAB Plant DNA Extraction USER/BILLING AGREEMENT?

The purpose of the CTAB Plant DNA Extraction USER/BILLING AGREEMENT is to establish a formal understanding between the provider of the extraction services and the user, ensuring clarity on the usage terms, billing cycle, and responsibilities of both parties.

What information must be reported on CTAB Plant DNA Extraction USER/BILLING AGREEMENT?

The information that must be reported on the CTAB Plant DNA Extraction USER/BILLING AGREEMENT includes user identification details, billing information, description of the intended use of the extracted DNA, compliance acknowledgments, and signature to confirm acceptance of the agreement terms.