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This document serves as a work order for the Microscopy Core in the Cellular & Molecular Physiology Department, detailing the information required to initiate a microscopy service request.

How to fill out microscopy core work order

How to fill out Microscopy Core Work Order

1. Access the Microscopy Core Work Order form online or obtain a physical copy.
2. Fill in your personal details including name, contact information, and lab affiliation.
3. Specify the type of microscopy service you require (e.g., fluorescence, electron microscopy).
4. Provide a detailed description of the samples you will be submitting.
5. Indicate the desired timeframe for completing the microscopy work.
6. Include any special instructions or requests for the microscopy service.
7. Review the form for accuracy and completeness before submission.
8. Submit the work order to the Microscopy Core office or through the designated online portal.

Who needs Microscopy Core Work Order?

1. Researchers needing microscopic analysis for their samples.
2. Students conducting experiments that require microscopy services.
3. Labs requiring specialized imaging techniques for their scientific work.
4. Anyone working on projects that involve biological, chemical, or materials analysis.

People Also Ask about

What is the pixel size of a confocal microscope?

Every pixel will be 5.86x5. 86 μm (375 μm/64=5.86). If you choose a scan format of 512x512 the pixel size will be reduced to 0.73 μm (375 μm/512=0.73).

What is the resolution of a confocal microscope?

Image resolution is typically 0.2 μm in the transverse plane (xy-plane) and ~ 0.5 μm in the z-plane. The greatest advantage is the possibility of making a 3D image of the surface of the sample within a depth of 100–200 μm. 9.1. Schematic of the confocal principle in epifluorescence scanning mode.

What is the resolution of a confocal microscope in NM?

Conventional microscopy techniques, namely the confocal microscope or deconvolution processes, are resolution limited, ~250 nm, by the diffraction properties of light as developed by Ernst Abbe in 1873.

What is the difference between fluorescent and confocal microscope?

The fluorescence microscope allows to detect the presence and localization of fluorescent molecules in the sample. The confocal microscope is a specific fluorescent microscope that allows obtaining 3D images of the sample with good resolution. In these microscopies, the sample contains fluorescent molecules.

What is confocal microscopy resolution?

Image resolution is typically 0.2 μm in the transverse plane (xy-plane) and ~ 0.5 μm in the z-plane. The greatest advantage is the possibility of making a 3D image of the surface of the sample within a depth of 100–200 μm. 9.1. Schematic of the confocal principle in epifluorescence scanning mode.

What is the overview of microscopy?

1 Introduction Microscopy is a technique used to visualize structures that cannot be observed with the eye. Its primary purpose is to form an image of the area intended to be observed.

What is the procedure of confocal microscopy?

In a confocal microscope, the illumination and detection optics are focused on the same diffraction-limited spot in the sample, which is the only spot imaged by the detector during a confocal scan. To generate a complete image, the spot must be moved over the sample and data collected point by point.

What is the resolution of a confocal Raman microscope?

For a true confocal design, typical spatial resolution is in the order of 0.5-1 µm.

For pdfFiller’s FAQs

What is Microscopy Core Work Order?

The Microscopy Core Work Order is a formal request document used to initiate microscopy services in a research facility, detailing specific imaging or analysis needs.

Who is required to file Microscopy Core Work Order?

Any researcher or staff member intending to utilize microscopy services must file a Microscopy Core Work Order.

How to fill out Microscopy Core Work Order?

To fill out the Microscopy Core Work Order, provide necessary details such as project title, sample information, required microscopy techniques, and contact information before submitting it to the core facility.

What is the purpose of Microscopy Core Work Order?

The purpose of the Microscopy Core Work Order is to streamline the request process for microscopy services, ensuring accurate tracking of requests and efficient allocation of resources.

What information must be reported on Microscopy Core Work Order?

The information that must be reported includes the researcher's name, department, project title, sample type, imaging requirements, deadlines, and any special instructions needed for the microscopy services.