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This form is used to submit requests for gene targeting injections at the Albert Einstein College of Medicine, including details about the principal investigator, knockout specifics, and charges for
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How to fill out Gene Targeting Facility Injection Form

01
Begin by entering your full name at the top of the form.
02
Provide your contact information, including phone number and email address.
03
Fill in the date of submission.
04
Specify the type of gene targeting service you require.
05
Include details about the specific gene or genes you wish to target.
06
Provide any relevant experimental background or context for your request.
07
Indicate the organism or model system you are working with.
08
Add any necessary references or prior research that supports your project.
09
Review all entries for accuracy and completeness.
10
Sign and date the form before submission.

Who needs Gene Targeting Facility Injection Form?

01
Researchers conducting gene targeting experiments.
02
Scientists working with genetic modifications in various organisms.
03
Institutions requiring assistance with gene manipulation.
04
Anyone needing specialized support from a Gene Targeting Facility.
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Gene targeting is a method that uses homologous recombination and embryonic stem cell technology to introduce mutations into a specific gene of interest. This technique relies on the capability to access known sequences of the mouse genome and the development of mouse embryonic stem cells.
Gene targeting is a method that uses homologous recombination and embryonic stem cell technology to introduce mutations into a specific gene of interest. This technique relies on the capability to access known sequences of the mouse genome and the development of mouse embryonic stem cells.
CRISPR/Cas9 is the most widely used genome editor and is a powerful tool for understanding gene function. Because CRISPR/Cas9 is an RNA-based system, it can be more efficiently and easily modified than the protein-based approaches and allows for targeting of multiple sites.
Gene targeting can be stimulated by induction of double-strand breaks at specific genomic sites. The expression of zinc finger nucleases (ZFNs) can induce genomic double-strand breaks.
In a typical cloning experiment, a target gene is inserted into a circular piece of DNA called a plasmid. The plasmid is introduced into bacteria via a process called transformation, and bacteria carrying the plasmid are selected using antibiotics.
The vector can be injected or given intravenously (by IV) directly into a specific tissue in the body, where it is taken up by individual cells. Alternately, a sample of the patient's cells can be removed and exposed to the vector in a laboratory setting.
Gene activation occurs when a stimulation, normally a physical-biological element, stimulates the natural proteins that are part of the genetic material of cells and is thus turning the genes “on” that were “off” and is therefore considered to be gene-regulating.
Researchers can use PCR as a method of searching for genes by using primers that flank the target sequence of the gene along with all other necessary components for PCR. If the gene is present, the primers will bind and amplify the DNA, giving a band of amplified DNA on the agarose gel that will be run.

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The Gene Targeting Facility Injection Form is a document used to request the injection of gene-targeted constructs into embryonic stem cells or other relevant cell types in gene targeting studies.
Researchers or laboratories that wish to utilize the services of a Gene Targeting Facility for gene injection activities must file the Gene Targeting Facility Injection Form.
To fill out the Gene Targeting Facility Injection Form, applicants need to provide detailed information about the project, including the gene targeting strategy, construct details, and any relevant safety information.
The purpose of the Gene Targeting Facility Injection Form is to ensure that all necessary information is captured to facilitate the successful execution of gene targeting experiments while adhering to safety and regulatory standards.
The form typically requires information such as the researcher's name, project title, gene targeting strategy, plasmid details, expected results, and any ethical considerations relevant to the study.
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