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Purity www. perkinelmer. com i.e. protein contamination is checked by determining the ratio of absorbance measurements at 260 nm and 280 nm. This can be further combined with a 320 nm background correction. Traditional methods for protein concentration measurement include colorimetric Bradford Lowry and BCA assays. These assays measure visible absorbance and are performed on standard clear microplates. UVcompatible plates and readers are not requ...

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